Human brain microvascular endothelial cells (HBMECs), cell culture media, and growth supplements were obtained from Sciencell (Carlsbad, CA, USA). Primary Antibodies against phospho-AMPKα (Thr-172), AMPKα, GAPDH, and all secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against occludin and anti-claudin-5 were from Invitrogen Life Technologies (Carlsbad, CA, USA). Antibodies against the NAD(P)H oxidase subunits (p47phox, p67phox) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). FITC-dextran was from Sigma–Aldrich (St. Louis, MO, USA). A stock solution of LPS (Escherichia coli O111:B4; Calbiochem, Germany) was prepared in PBS. The concentration of LPS was selected to mimic sepsis [17 (link)].
Cell culture media
Manufactured by ScienCell
Sourced in United States
Cell culture media is a liquid or powder formulation that provides essential nutrients, growth factors, and other components necessary for the growth and maintenance of cells in a laboratory setting. It serves as the fundamental substrate for cell culture experiments and research.
Automatically generated - may contain errors
2 protocols using cell culture media
1
Brain Microvascular Endothelial Cell Culture
2
Investigating ApoE Isoforms and BDNF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture media were obtained from Sciencell, CA, USA. Recombinant human ApoE2 (rh-ApoE2), ApoE3 (rh-ApoE3), ApoE4 (rh-ApoE3), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies against BDNF and β-actin were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); all secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (Bar Harbor, ME, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!