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3 protocols using benzo a pyrene

1

Nuclease and Phosphatase Enzyme Purchases

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Bovine spleen phosphodiesterase II (SPD) and micrococcal nuclease (MN) were purchased from Worthington Biochemical Corp. (Lakewood, NJ, USA). Bacterial alkaline phosphatase Type III (E. coli), 2′-deoxycytidine, 2′-deoxythymidine, 2′-deoxyadenosine, 2′-deoxyGuanosine and 2′,3′-dideoxyinosine were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Benzo[a]pyrene (>98%), adenosine (>98%), phosphate buffered saline(-) [PBS(-)], methanol (LC/MS grade), and dimethyl sulfoxide (DMSO) were purchased from Wako Chemical (Osaka, Japan). Guanosine (>98%), cytidine (>98%) and uridine (>98%) were purchased from Tokyo Chemical Industry Co (Tokyo, Japan).
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2

PAH Extraction and Quantification

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Hexane, acetone, ethanol, and sodium sulfate (Wako Pure Chemicals Industries, Ltd., Japan) used for the analyses were of residual agricultural chemical grade. Water purified by a Millipore Direct-Q® 3UV water purification system was washed with Hexane twice prior to use. Authentic PAH standards (phenanthrene [Phe], anthracene [Anth], fluoranthene [Flu], pyrene [Pyr], chrysene [Chry], benzo[b]fluoranthene [[b]flu], benzo[a]pyrene [[a]pyr], and perylene [Pery]), deuterated PAHs, and p-terphenyl-d14 were purchased from Wako Pure Chemicals Industries, Ltd. (Japan). Deuterated surrogate standards were used to compensate for PAH losses during analysis.
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3

Benzo[a]pyrene Sorption Kinetics in Peat

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Benzo[a]pyrene (>97.0% purity, Chemical Abstracts Service [CAS] no. 50‐32‐8), CPS (>99.0% purity, CAS no. 2921‐88‐2), Phe (>99.0% purity, CAS no. 85‐01‐8), Pyr (>98.0% purity, CAS no. 129‐00‐0), acetone (>99.5% purity), acetonitrile (>99.8% purity), and kaolin (practical grade) were purchased from FujiFilm Wako Pure Chemicals. Chlorpyrifos‐d10 was purchased from Kanto Chemical. Quartz sand (0.2–0.8 mm particle ≥40%, loss on ignition at 900 °C ≤0.05%) was purchased from Merck. Peat was purchased form Midori, which was collected from Hokkaido, Japan. The elemental composition of the peat as determined by an elemental analyzer (Flash EA1112; Thermo Fisher Scientific) was as follows: N 1.5% w/w, C 37.6% w/w, H 4.4% w/w.
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