NMDAR, p-CREB, and CD11b expression in the striatum was evaluated by immunofluorescence. Briefly, the brain tissue was frozen and the mid-part of the striatum was cut into 20 μm slices in the coronal plane. The slices were washed three-times in PBS (pH 7.4) for 5 min each time, blocked with 10% goat serum, and incubated with the following primary antibodies overnight at 4 °C: anti-phospho-CREB (1:1000, Abcam), NMDAR (1:1000, Abcam), and CD11b (1:1000, Abcam). The slices were then incubated with goat anti-rabbit secondary antibody (1:500, Beyotime) for 2 h in the darkness at room temperature, and washed three-times in PBS for 10 min each time. Images were captured using a fluorescence microscope (IX71; Olympus Corporation, Tokyo, Japan).
Nmdar
Manufactured by Abcam
Sourced in United States
The NMDAR is a subunit of the NMDA (N-Methyl-D-Aspartate) receptor, which is an ionotropic glutamate receptor involved in excitatory neurotransmission in the central nervous system. The NMDAR plays a crucial role in synaptic plasticity, learning, and memory processes.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit secondary antibody, by Beyotime (1 mentions)
Anti phospho creb, by Abcam (1 mentions)
Cd11b, by Abcam (1 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
Gapdh, by Abcam (1 mentions)
Cyclin a2, by Abcam (1 mentions)
Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions)
Cyclin d1, by Abcam (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using nmdar
1
Striatal Immunofluorescence Analysis
2
Antibody Validation for Cellular Analysis
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PCSO-524® was a generous gift from PharmaLink Company (Abu Dhabi, UAE). Antibodies against cyclin D1 (diluted 1:1000), cyclin A2 (diluted 1:1000), GAPDH (diluted 1:25,000), NMDAR (diluted 1:1000), and MAP2 (diluted 1:5000), were purchased from Abcam (Cambridge, MA, USA); PCNA (diluted 1:2000), antibody was purchased from Cell Signaling Technology (Danvers, MA, USA); Synapsin1 (diluted 1:20,000) and NeuN (diluted 1:3000) antibodies were purchased from Merck Millipore (Burlington, NJ, USA). Secondary antibody conjugated with fluorescent Alexa dye 488, 647 and Cy3 were purchased from Invitrogen (Carlsbad, CA, USA) and The Jackson Laboratory. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Invitrogen.
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