Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), L-lysine, phosphate buffer solution (PBS) tablets, sodium chloride (NaCl), sodium acetate and rhodamine B were all purchased from Sigma-Aldrich (Castle Hill, NSW Australia). N-hexane, dimethyl sulfoxide (DMSO), methanol (MeOH), hydrochloric acid (HCl) and acetic acid were sourced from ChemSupply (Gillman, SA Australia).
Dimethyl sulfoxide (dmso)
Manufactured by Chem-Supply
Sourced in Australia
Dimethyl sulfoxide (DMSO) is a colorless, odorless, and water-soluble organic compound. It is a powerful polar aprotic solvent that is commonly used in various laboratory applications. DMSO has a high boiling point and is known for its ability to penetrate the skin and other biological membranes.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Chem-Supply (2 mentions)
Sep pak c18 column, by Waters Corporation (2 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by Merck Group (2 mentions)
Potassium iodide ki, by Merck Group (2 mentions)
Milli q water, by Merck Group (2 mentions)
Hydrogen peroxide h2o2, by Avantor (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
Acetic acid, by Chem-Supply (1 mentions)
N hexane, by Chem-Supply (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
L lysine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Chem-Supply (1 mentions)
Nile red, by Merck Group (1 mentions)
Methanol meoh, by Chem-Supply (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Rat tail collagen, by Merck Group (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
6 protocols using dimethyl sulfoxide (dmso)
1
Synthesis of Rhodamine B-Doped Silica Nanoparticles
2
Visualizing Triacylglycerol in Cream
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Cream samples were stained with Nile red (Sigma-Aldrich, St. Louis, MO) so that observe the triacylglycerol core of fat globules. The Nile red solution (1 mg/mL) was prepared in dimethyl sulfoxide (Chem Supply, Gillman, Australia) and kept protected from light until use. Cream samples were initially diluted 1:5 with water at room temperature. Then, 900 µL of sample was mixed with 100 µL of Nile red solution and incubated for 15 min at room temperature. An inverted confocal laser scanning microscope from Leica Microsystem (Heidelberg, Germany) was used, adopting the conditions described by Ong et al. (2010) . Nile red was excited at 488 nm using an argon laser and the emission filter was set at 520 to 590 nm.
3
Synthesis and Characterization of Platinum(II) Complexes
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All reagents were used as received without further purification. MilliQTM water (Merck, Melbourne, Australia) was utilised, and organic solvents were of analytical grade. We acquired 1S,2S-Diaminocyclohexane (SSDACH, 98%), 4,7-dimethoxy-1,10-phenanthroline (47O2Me2Phen), 1,10-phenanthroline (Phen), 5-methyl-1,10-phenanthroline (5MePhen), 5,6-dimethyl-1,10-phenanthroline (56Me2Phen), biotin, silver nitrate, N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC-HCl), and N-hydroxysuccinimide (NHS) from Sigma-Aldrich, Sydney, NSW. Potassium tetrachloroplatinate (K2PtCl4) was obtained from Precious Metals Online. Potassium iodide (KI) was purchased from Merck, Melbourne, Australia. Hydrogen peroxide (H2O2, 30%) was obtained from VWR chemicals, Philadelphia, USA. Acetonitrile (ACN), dimethyl formamide (DMF), diethyl ether, methanol (MeOH), and dimethyl sulfoxide (DMSO) were purchased from Chem-Supply, Adelaide, Australia. Sep-Pak® C18 columns were acquired from Waters, Australia. For ICP-MS experiments, the certified reference standard used was purchased from High-Purity standards, North Charleston, United States. Ultra-pure HNO3 (69%) was obtained from Choice Analytical, Sydney, Australia. Deuterated solvents used for NMR experiments (d6-dimethyl sulfoxide (DMSO-d6, 99.9%) and deuterium oxide (D2O, 99.9%) were acquired from Novachem, Melbourne, Australia.
4
Reagents for Cell Culture and Analysis
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RPMI-1640, DMEM, foetal bovine serum (FBS), trypsin, penicillin/streptomycin solution, G418 sulfate, sodium pyruvate and glutamine were obtained from Invitrogen (Life Technologies, VIC, Australia). Non-essential amino acids (NEAA) were from Lonza (VIC, Australia). IGEPAL, leupeptin, aprotinin, 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT), hematoxylin, rat tail collagen and nitrocellulose membranes were purchased from Sigma-Aldrich (NSW, Australia). Dimethyl sulfoxide (DMSO) was from Chem-supply (SA, Australia). Immobilon polyvinyldifluoride (PVDF) membranes were from Fisher Scientific (VIC, Australia). Random primers and Recombinant RNasin® Ribonuclease inhibitors were obtained from Promega (NSW, Australia). Rabbit polyclonal anti-caveolin-1 was from BD Biosciences (NSW, Australia), anti-PTRF from ProteinTech (NSW, Australia), anti-VEGF-A was from R&D (BioScientific, NSW, Australia), mouse monoclonal antibody to GAPDH was from Acris (Life Research, NSW, Australia).
5
Synthesis and Characterization of Platinum(II) Complexes
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All reagents were used as received unless otherwise specified, all organic solvents utilised were of analytical grade, and MilliQTM water (Merck, Melbourne, Australia) was used. Potassium tetrachloroplatinate (K2PtCl4) was acquired from Precious Metals Online. 1S,2S-Diaminocyclohexane (SSDACH, 98%), 1,10-phenanthroline (Phen), 5,6-dimethyl-1,10-phenanthroline (56Me2Phen), silver nitrate, N,N′-dicyclohexylcarbodiimide (DCC), N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC-HCl), N-hydroxysuccinimide (NHS), indomethacin and aspirin were purchased from Sigma-Aldrich, Sydney, NSW. Sep-Pak® C18 columns were obtained from Waters, Australia. Methanol, diethyl ether, ethyl acetate, dichloromethane (DCM), dimethyl formamide (DMF), dimethyl sulfoxide (DMSO) and acetonitrile (ACN) were acquired from Chem-Supply, Adelaide, Australia. Hydrogen peroxide (H2O2) (30%) was purchased from VWR chemicals, Philadelphia, United States. Potassium iodide (KI) was obtained from Merck, Melbourne, Australia. Ultra-pure HNO3 (69%) was acquired from Choice Analytical, Sydney, Australia. The certified reference standard that was used for the ICP-MS experiments was purchased from High-Purity Standards, North Charleston, United States. The deuterated solvents deuterium oxide (D2O, 99.9%) and d6-dimethyl sulfoxide (DMSO-d6, 99.9%) were obtained from Novachem, Melbourne, Australia.
6
Fabrication of Fluorescent Nanoparticles for Brain Endothelial Cell Imaging
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Cetyltrimethylammonium bromide (CTAB), Tetraethoxysilane (TEOS), ammonium hydroxide, 3-Aminopropyltriethoxysilane (APTES), FITC-Carboxymethyl dextran 70 kD, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Melbourne, Australia). Dimethyl sulfoxide (DMSO) was bought from Chem-supply (Adelaide, Australia). Cyanine 5 NHS ester (Cy5) dye was purchased from Lumiprobe Limited (Cockeysville, MD, USA, Asia & Pacific). Dulbecco’s Modified Eagle Medium (DMEM), penicillin/streptomycin (P/S), and 0.25% trypsin-EDTA were bought from Life Technologies (Brisbane, Australia). (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) buffer was obtained from Gibco Australia. Pure ethanol was purchased from the University of Queensland Science store.
The human brain endothelial cell line (hCMEC/D3) (lot#SCC066) was obtained from Millipore Merck (Melbourne, Australia). Alamar Blue Assay Kit and cell staining dye were bought from Thermofisher (Brisbane, Australia) and included: DAPI (4′,6-diamidino-2-phenylindole), Alexa Fluor 594 conjugated anti-Zonula occludens-1 (ZO-1) antibodies, and Alexa Fluor 488 conjugated anti-Claudin 5 antibodies.
The human brain endothelial cell line (hCMEC/D3) (lot#SCC066) was obtained from Millipore Merck (Melbourne, Australia). Alamar Blue Assay Kit and cell staining dye were bought from Thermofisher (Brisbane, Australia) and included: DAPI (4′,6-diamidino-2-phenylindole), Alexa Fluor 594 conjugated anti-Zonula occludens-1 (ZO-1) antibodies, and Alexa Fluor 488 conjugated anti-Claudin 5 antibodies.
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