Cells were washed twice with ice-cold PBS and ruptured with CLB buffer (Cell Signaling Technology) containing PMSF and cocktail inhibitor. Cell lysates were resolved by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA) and then blotted. Specific antibodies used are listed below: anti-Mettl3 antibody (15073–1-AP, 1:500) was from Proteintech; anti-DDIT4 antibody (1:1000) was from Proteintech; antibody to IkBα phosphorylated at Ser32 (2859S, 1:1000), anti-IkBα antibody (9234S, 1:3000), antibody to P70S6K phosphorylated at T389 (9234S, 1:1000), antibody to AKT phosphorylated at T308 (4056S, 1:1000), antibody to p65 phosphorylated at Ser536 (3031S, 1:1000), anti-NFkB p65 antibody (6956S, 1:1000), anti-ATG5 antibody (12994S, 1:1000), anti-β-actin (3700S, 1:10000), anti-Flag-HRP (2044S, 1:20000) were from Cell Signaling Technology. All of the unprocessed scans of the blots were shown in the Source Data file.
Anti mettl3 antibody 15073 1 ap
Manufactured by Proteintech
Sourced in United States
The Anti-Mettl3 antibody (15073-1-AP) is a primary antibody produced by Proteintech. It is designed to detect the Mettl3 protein, which is an enzyme involved in the methylation of RNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
Anti atg5 antibody, by Cell Signaling Technology (2 mentions)
Anti flag hrp, by Cell Signaling Technology (2 mentions)
Anti nf kb p65 antibody, by Cell Signaling Technology (2 mentions)
Clb buffer, by Cell Signaling Technology (2 mentions)
Anti ddit4 antibody, by Proteintech (2 mentions)
Anti ikbα antibody, by Cell Signaling Technology (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Cell counting kit 8 (cck8), by Solarbio (1 mentions)
Actinomycin d, by Merck Group (1 mentions)
3 protocols using anti mettl3 antibody 15073 1 ap
1
Western Blot Analysis of Signaling Pathways
2
Western Blot Analysis of Signaling Pathways
3
Bortezomib Inhibits Circular RNA
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Bortezomib (SB8980) and Cell Counting Kit-8 (CK04) were obtained from Solarbio (Beijing, China). Animal Total RNA Isolation Kit (B518621), AMV One-step RT-PCR Kit (B532441), Dual Luciferase Reporter Gene Assay Kit (E608001), and primer were purchased from Sangon Biotech (Shanghai) Co., Ltd. circ_0000337 high-expressed and knock-down plasmid were purchased from GENECHEM (Shanghai, China). Anti-DNA2 antibody (ab96488), Anti-GAPDH antibody (ab8245), Anti-YTHDF1 antibody (17479-1-AP), Anti-YTHDF2 antibody (24744-1-AP), Anti-METTL3 antibody (15073-1-AP), and Anti-FTO antibody (27226-1-AP) are available from Proteintech (United States). Actinomycin D is purchased from Merck KGaA (Germany). DMEM (SH30249.02), RPMI 1640 (SH30027.01), fetal bovine Serum (SH30084.03), penicillin streptomycin (SV30010), and trypsin (SH30042.01) were purchased from HyClone (United States). Serum-free cell freeze (C40100) is produced by NCM Biotech (Suzhou, China).
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