In experimental week 5, 129SV/pas mice were sedated with 90 mg/kg ip pentobarbital (APL), and basal O2 consumption was measured for 30 min at 33°C in the temperature-controlled metabolic chambers. Subsequently, the mice were taken out of the chambers, and 1 mg/kg norepinephrine bitartrate salt monohydrate (NE; Sigma) was injected subcuteanously. O2 consumption was then measured for another 60–80 min. The average of the basal O2 consumption was set to zero, and the O2 consumption trace was expressed as O2 consumption minus average basal O2 consumption. UCP1-dependent O2 consumption was calculated by subtracting the average O2 consumption trace of the UCP1-KO mice from the average O2 consumption trace of the WT mice per diet group.
Norepinephrine bitartrate salt monohydrate ne
Manufactured by Merck Group
Sourced in United States
Norepinephrine bitartrate salt monohydrate (NE) is a chemical compound used in laboratory settings. It is a salt form of the neurotransmitter norepinephrine, which is also known as adrenaline. NE is commonly used in research and experimental applications to study the physiological effects of norepinephrine in various biological systems.
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Lab products found in correlation
Isoproterenol hydrochloride, by Merck Group (1 mentions)
Coelenterazine 400a, by Interchim (1 mentions)
Epinephrine epi, by Merck Group (1 mentions)
Coelenterazine h, by Interchim (1 mentions)
Acetylcholine chloride, by Merck Group (1 mentions)
Acetate salt hydrate, by Merck Group (1 mentions)
6 hydroxydopamine hydrochloride, by Merck Group (1 mentions)
6 ohda, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
L name, by Merck Group (1 mentions)
Corticosterone, by Merck Group (1 mentions)
4 protocols using norepinephrine bitartrate salt monohydrate ne
1
Norepinephrine-Stimulated Oxygen Consumption
2
Cardiovascular Receptor Agonist Assay
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(−)-Isoproterenol hydrochloride (ISO), (−)-norepinephrine (+) bitartrate salt monohydrate (NE), (−)-epinephrine (EPI), and angiotensine II (AngII) were purchased from Sigma-Aldrich (St. Louis, MO, USA) while coelenterazine 400a and coelenterazine h were purchased from Interchim.
3
Cardiovascular Pharmacology Protocol
4
Investigating Metabolic Responses to Adrenergic Stimuli
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Male 10-week-old C57Bl6/J mice were acclimated to 30 C for two weeks. When the animals were 12 weeks old, they were sedated with 90 mg/kg pentobarbital (APL, Sweden) i.p. and baseline oxygen consumption was measured at 30 C in metabolic chambers (INCA system, Somedic, Sweden) . Measurements were obtained every second minute. The mice were then briefly taken out of the chambers and acutely injected i.p. with vehicle (5% EtOH in sterile saline), 1 mg/kg norepinephrine bitartrate salt monohydrate (NE, Sigma, Germany), or 0.3 mg/kg, 3 mg/kg or 30 mg/kg corticosterone (Sigma, Germany), after which O 2 consumption measurements were continued.
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