Purified NanoLuc (Promega), was distributed in a 96-well half-area white microplate (Costar, CLS3693) to a final concentration of 10 pmol/L in PBS; PBS, 0.1% BSA; 50% Nano-Glo assay buffer (Promega, N112B) in PBS or 50% Nano-Glo assay buffer, 0.1% BSA with two different dilutions (1/100 and 1/400 final) of furimazine (Nano-GloTM assay substrate, Promega, N113B). After a short centrifugation (500 rpm, 1 min) to gather reagent in the bottom of wells, luminescence was read (0.1 s/well, without filter) on a microplate reader (Mithras LB940, Berthold Technologies, Germany) after different incubation times at room temperature.
Nano glo assay buffer
Manufactured by Promega
The Nano-Glo assay buffer is a component designed for use with Promega's Nano-Glo Luciferase Assay System. The buffer provides the necessary reagents to facilitate the luminescent reaction that occurs between the Nano-Glo luciferase enzyme and its substrate. This enables the quantification of luciferase activity in various experimental settings.
Automatically generated - may contain errors
3 protocols using nano glo assay buffer
1
NanoLuc Luminescence Assay Protocol
2
Quantifying HAV Infection by Luciferase
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were plated in 96-well plates and infected the next day with equal amounts of HAV-Nluc. Lysates were harvested by washing once with PBS wash followed by addition of 22 μl of Passive Lysis Buffer (Promega) under shaking for 15 min. For luminescence readout, 15 μl of lysate were mixed with 50 μl of Nano-Glo assay buffer (Promega) in a flat bottom white-walled luminescence plate, incubated for 5 min at room temperature and read on an EnVision plate reader (PerkinElmer).
3
Quantifying HAV Infection by Luciferase
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