Plasma samples were analyzed for 51 plasma cytokines and angiogenic factors: TGF-β1, TGF-β2, TGF-β3, IFN-α2, IL-1α, IL-2Rα, IL-3, IL-12p40, IL-16, IL-18, CTACK, Gro-α, HGF, LIF, MCP-3, M-CSF, MIF, MIG, β-NGF, SCF, SCGF-β, SDF-1α, TNF-β, TRAIL, IL-1β, Il-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Eotaxin, FGF basic, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF bb, RANTES, TNF-α, VEGF using predesigned panels as described previously and were available for subset of patients (Bio-Plex Pro TGF-β assay, Bio-Plex Pro Human Cytokine 21- and 27-plex immunoassays; Bio-Rad Laboratories, Hercules, CA, USA) [22 (link)]. The large panel of cytokines was analyzed as data were available from the previous study [22 (link)].
Bio plex pro human cytokine 21 and 27 plex immunoassays
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Pro Human Cytokine 21- and 27-plex immunoassays are multiplexed bead-based assays designed to measure the concentrations of up to 21 or 27 different human cytokines, chemokines, and growth factors simultaneously in a single sample. These assays leverage the Luminex xMAP technology and provide a quantitative analysis of the target analytes.
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2 protocols using bio plex pro human cytokine 21 and 27 plex immunoassays
1
Cytokine and Angiogenic Factor Profiling
2
Plasma Biomarker Analysis Protocol
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Plasma uPA and plasminogen activator inhibitor-1 (PAI-1) were analyzed using enzyme-linked immunosorbent assays (ELISA) as described previously (35 (link)). Briefly, plasma tissue factor (TF) was analyzed by ELISA using the Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay (R&D Systems, Minneapolis, MN, USA). D-dimer in plasma samples was determined using the IMUCLONE D-Dimer ELISA (American Diagnostica, Greenwich, CT, USA) system. Plasma uPA was measured using the Human u-Plasminogen Activator/Urokinase Quantikine ELISA kit (R&D Systems). Plasma PAI-1 was measured using the Human Serpin E1/PAI-1 Quantikine ELISA Kit (R&D Systems).
Plasma samples were analyzed for 51 plasma cytokines and angiogenic factors (TGF-β1, TGF-β2, TGF-β3, IFN-α2, IL-1α, IL-2Rα, IL-3, IL-12p40, IL-16, IL-18, CTACK, Gro-α, HGF, LIF, MCP-3, M-CSF, MIF, MIG, β-NGF, SCF, SCGF-β, SDF-1α, TNF-β, TRAIL, IL-1β, Il-1RA, IL-2, IL-4, IL-5,IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, FGF basic, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF bb, RANTES, TNF-α, and VEGF) using pre-designed panels as described previously and were available for subset of patients (Bio-Plex Pro TGF-β assay, Bio-Plex Pro Human Cytokine 21- and 27-plex immunoassays; Bio-Rad Laboratories, Hercules, CA, USA) (35 (link)).
Plasma samples were analyzed for 51 plasma cytokines and angiogenic factors (TGF-β1, TGF-β2, TGF-β3, IFN-α2, IL-1α, IL-2Rα, IL-3, IL-12p40, IL-16, IL-18, CTACK, Gro-α, HGF, LIF, MCP-3, M-CSF, MIF, MIG, β-NGF, SCF, SCGF-β, SDF-1α, TNF-β, TRAIL, IL-1β, Il-1RA, IL-2, IL-4, IL-5,IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, FGF basic, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF bb, RANTES, TNF-α, and VEGF) using pre-designed panels as described previously and were available for subset of patients (Bio-Plex Pro TGF-β assay, Bio-Plex Pro Human Cytokine 21- and 27-plex immunoassays; Bio-Rad Laboratories, Hercules, CA, USA) (35 (link)).
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