Mulberroside C (Figure 1 ) was provided by ChemFaces (Wuhan, China). Mouse monoclonal to CD62P (P-selectin) antibody was purchased from Biolegend (San Diego, CA, USA). Fura 2-AM (2-acetoxymethyl) and Alexa Fluor 488-conjugated fibrinogen were obtained from Invitrogen (Eugene, OR, USA). The BCA protein assay kit was obtained from Pierce Biotechnology (Rockford, IL, USA). The serotonin ELISA kit was provided by Labor Diagnostika Nord GmbH and Co. (Nordhorn, Germany). Collagen, U46619, and thrombin were purchased from Chrono-Log Co. (Havertown, PA, USA). The thromboxane B2 assay kit, cAMP, cGMP enzyme immunoassay kit, and ATP assay kit were obtained from Cayman Chemical (Ann Arbor, MI, USA). Cell signaling (Beverly, MA, USA) supplied antiphospho-p38MAPK, antiphospho-ERK (1/2), antiphospho-VASP (Ser157), antiphospho-VASP (Ser239), antiphospho-cPLA2 (Ser505), antiphospho-PI3K (Tyr458), antiphospho-Akt (Ser473), antiphospho-inositol-3-phosphate receptor type I (Ser1756), antiphospho-PLCγ2 (Tyr759), anti-β-actin, and antirabbit secondary antibodies.
Fura 2 am 2 acetoxymethyl
Manufactured by Thermo Fisher Scientific
Sourced in United States
Fura 2-AM (2-acetoxymethyl) is a fluorescent calcium indicator used for measuring intracellular calcium concentrations. It is a cell-permeant form of the Fura-2 dye that can be loaded into cells and then undergoes changes in its fluorescence properties in response to changes in calcium levels.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 conjugated fibrinogen, by Thermo Fisher Scientific (3 mentions)
Thrombin, by Chrono-Log (3 mentions)
Anti phospho p38 mapk, by Cell Signaling Technology (2 mentions)
Cgmp enzyme immunoassay kit, by Cayman Chemical (2 mentions)
Thromboxane b2 assay kit, by Cayman Chemical (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
U46619, by Chrono-Log (2 mentions)
Anti phospho pi3k, by Cell Signaling Technology (1 mentions)
Atp assay kit, by Cayman Chemical (1 mentions)
Anti phospho cpla2, by Cell Signaling Technology (1 mentions)
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Mouse monoclonal to cd62p p selectin antibody, by BioLegend (1 mentions)
Antiphospho vasp, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Thromboxane b2, by Cayman Chemical (1 mentions)
Atp detection kit, by Cayman Chemical (1 mentions)
Thrombin from human plasma, by Merck Group (1 mentions)
U46619, by Cayman Chemical (1 mentions)
Bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions)
Camp eia, by Cayman Chemical (1 mentions)
5 protocols using fura 2 am 2 acetoxymethyl
1
Mulberroside C Signaling Pathway Analysis
2
Platelet Signaling Pathway Analysis
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The supply of 5-hydroxyindolin-2-one (5-HI) was purchased from ChemFaces (Wuhan, China). Fura 2-AM (2-acetoxymethyl) and fibrinogen (Alexa Fluor 488 conjugated) were purchased from Invitrogen (Eugene, OR, USA). A serotonin detection kit was purchased from Labor Diagnostika Nord GmbH and Co. (Nordhorn, Germany). Physiological agonists (collagen, U46619, and thrombin) were obtained from Chrono-Log Co. (Havertown, PA, USA). Cell Signaling (Beverly, MA, USA) supplied anti-phospho-p38MAPK, anti-phospho-inositol-3-phosphate receptor type I (Ser1756), anti-phospho-ERK (1/2), anti-phospho-VASP (Ser157), anti-phospho-cPLA2 (Ser505), anti-phosphor-PI3K (Tyr458), anti-phospho-Akt (Ser473), anti-β-actin, and anti-rabbit secondary antibodies. The thromboxane B2, cAMP, and ATP detection kit were purchased from Cayman Chemical (Ann Arbor, MI, USA).
3
Platelet Activation Pathway Analysis
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Collagen, ADP, and thrombin were obtained from Chrono-Log Co. (Collagen cat # 385 ADP cat #384 thrombin cat #386, CHRON-LOG Corporation, Havertown, PA, USA). Paraformaldehyde (CAS no. 30525-89-4) and glutaraldehyde (CAS no. 111-30-8) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The ATP Assay kit was obtained from the Biomedical Research Service (REF. 373, BMR, 3434 Main St, Buffalo, NY, USA). thrombin from human plasma was purchased from Sigma-Aldrich (Lot # SLCF9776 CAS no. 9002-04-4 647-014-00-9). Fura 2-AM (2-acetoxymethyl) (CAS number: 108964-32-5) and Alexa Fluor 488-conjugated fibrinogen (Cat # A32723) were obtained from Invitrogen (Eugene, OR, USA). All antibodies were supplied by Cell Signaling (Beverly, MA, USA).
4
Platelet Activation and Signaling Assays
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Derrone was purchased from ChemFaces (Wuhan, China). Collagen was purchased from Chrono-Log Co. (Havertown, PA, USA). Fura 2-AM (2-acetoxymethyl) and Alexa Fluor 488-conjugated fibrinogen were obtained from Invitrogen (Eugene, OR, USA). Serotonin ELISA kit was purchased from Labor Diagnostika Nord GmbH and Co. (Nordhorn, Germany). Bicinchoninic acid protein assay kit was purchased from Pierce Biotechnology (IL, USA). Cayman chemical (Ann Arbor, MI, USA) offered thromboxane B2 assay kit, cAMP, cGMP enzyme immunoassay kit, and U46619. Cell Signaling (Beverly, MA, USA) supplied all antibodies. The adhesion kit (fibronectin coated) was obtained from Cell Biolabs (San Diego, CA, USA).
5
Platelet Activation and Signaling Assays
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ChemFaces (Wuhan, China) supplied EFA. The Chrono-Log corporation (Havertown, PA, USA) supplied platelet collagen. Cayman Chemical (Ann Arbor, MI, USA) supplied the cAMP EIA and thromboxane B 2 assay kits. Cell Signaling (Beverly, MA, USA) supplied the lysis buffer and antibodies against phosphovasodilator-stimulated phosphoprotein (VASP), phospho-inositol-3-phosphate receptor type I (IP 3 RI), phospho-cytosolic phospholipase A 2 (cPLA 2 ), phospho-p38 mitogen-activated protein kinase (p38 MAPK ), phospho-Akt, and â-actin, and anti-rabbit secondary antibodies. Invitrogen (Eugene, OR, USA) provided Fura 2-AM-(2-acetoxymethyl-) and Alexa Fluor 488-conjugated fibrinogen. The fibronectin-coated cell adhesion kit was procured from Cell Biolabs (San Diego, CA, USA). The serotonin enzyme-linked immunosorbent assay (ELISA) kit was purchased from Labor Diagnostika Nord GmbH & Co. (Nordhorn, Germany).
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