Cd4 clone rm4 4

Manufactured by BD

Sourced in Germany

CD4 (clone RM4-4) is a monoclonal antibody that binds to the CD4 molecule. CD4 is a cell surface glycoprotein that serves as a co-receptor for the T-cell receptor. The CD4 (clone RM4-4) antibody is commonly used in flow cytometry applications to identify and quantify T helper cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CD4 (RM4-4), (2 citations)

3 protocols using cd4 clone rm4 4

Comprehensive Murine Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorochrome-conjugated antibodies specific for CD11c (clone N418), CD11b (clone M1/70), Ly-6C (clone HK1.4), Ly-6G (clone 1A8), MHCII I-A/I-E (clone M5/114.15.2), CD90.1 (clone HIS51), CD3 (clone 17A2), iNOS (clone CXNFT), IL12-p40 (clone C17.8), CD19 (clone eBio1D3), IL-2 (clone JES6-5H6), CD86 (clone GL1), CD27 (clone LG.7F9), NK1.1 (clone PK136), CD49b (clone DX5), NKp46 (clone 29A1.4), CD45.1 (clone A20), CD45.2 (clone 104), IFN-γ (clone XMG1.2), (eBioscience, San Diego, CA) CD4 (clone RM4-4), CD8α (clone 53-6.7), TNF (clone MP6-XT22) (BD Bioscience) and XCR1 (clone ZET) (Biolegend, San Diego, CA) were used at optimal titers as determined in our laboratory.
Serum cytokines were determined using the Mouse Inflammation BD Cytometric Bead Array (CBA, BD Biosciences, San Jose, CA). Samples were acquired on an LSRII flow cytometer and the exported data were analyzed using the CBA Analysis Plugin for Excel.

Alice A.F., Kramer G., Bambina S., Baird J.R., Bahjat K.S., Gough M.J, & Crittenden M.R. (2017). Amplifying IFNγ signaling in DC by CD11c-specific loss of SOCS1 increases innate immunity to infection while decreasing adaptive immunity. Journal of immunology (Baltimore, Md. : 1950), 200(1), 177-185.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions from spleen, peritoneal lavage, uterus, blood, inguinal (ILN) and para-aortic lymph nodes (PLN) were obtained and stained for cell surface markers for 30 min at 4 °C. The following anti-mouse fluorescently labeled antibodies were used: CD19 (clone 1D3) and CD4 (clone RM4-4; both from BD Biosciences, Germany), CD25 (clone 3C7), IgM (clone RMM-1), IgD (clone 11-26 c.2a) and B220 (clone RA3-6B2; all from Biolegend, San Diego, CA, USA). For detection of the intracellular expression of Foxp3, cell suspensions were fixed ON using Fix and Perm (ebioscience, Germany) and stained with anti-mouse Foxp3 (clone FJK-16s; ebioscience) for 30 min at 4 °C. Measurements were performed with a FACSCalibur and analyzed with CellQuestPro software (BD Biosciences).

Busse M., Campe K.N., Nowak D., Schumacher A., Plenagl S., Langwisch S., Tiegs G., Reinhold A, & Zenclussen A.C. (2019). IL-10 producing B cells rescue mouse fetuses from inflammation-driven fetal death and are able to modulate T cell immune responses. Scientific Reports, 9, 9335.

+ Open protocol

+ Expand

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lymphocytes were counted and subjected to viability staining (Fixable Aqua Dead Cell staining, Life Technologies, #L34957) and subsequently to receptor Fc blockade (BD #553142). Staining was performed using the following antibodies: CD4 (clone RM4-4, BD), CD45 (clone 30-F11 eBioscience), CD45.1 (clone A20, BD), CD45.2 (clone 104, BD) CD8a (clone 53-6.7, eBioscience or BD), CD8b (clone YTS156.7.7, BioLegend), CD11b (clone RM2817, Thermo Fisher), Ly6c (clone AL-21, BD), CD3ε (clone 145-2C11, BD), CD19 (clone 1D3, BD), CD90.2 (53-2.1, BD), CD127 (clone A7R34, BD), CX3CR1 (clone SA011F11, BioLegend), CXCR3 (clone CXCR3-173, Biolegend), CD103 (clone 2E7, eBioscience or Biolegend), CD69 (clone H1.2F3, BD), TCR-β (clone H57-597, BD, TCR-γδ (clone eBio-GL3, eBioscience). H2-K^b:SIINFEKL or H2-M3:fMIGWII tetramers were either produced in house or, for the former, obtained thorugh the NIH tetramer core. Samples were fixed (IC Fixation Buffer, eBioscience), washed, resuspended in FACS buffer and acquired with a LSRII flow cytometer (BD) either immediately or on the following day. For intracellular staining, the following antibodies were used: IFN-ϒ (clone XMG1.2, BD), TNF-α (clone MP6-XT22, BD) in Permeabilization buffer (eBioscience).

Becattini S., Littmann E.R., Seok R., Amoretti L., Fontana E., Wright R., Gjonbalaj M., Leiner I.M., Plitas G., Hohl T.M, & Pamer E.G. (2020). Enhancing mucosal immunity by transient microbiota depletion. Nature Communications, 11, 4475.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!