Lipase

Manufactured by Solarbio

Sourced in China

Lipase is a type of enzyme that catalyzes the hydrolysis of lipids, such as triglycerides, into fatty acids and glycerol. It is commonly used in biochemical and analytical applications.

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Lipase activity assay kit (5 citations) Pancreatic lipase (2 citations)

6 protocols using lipase

Fibrin-based Tissue Engineering Protocol

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PU was purchased from Sigma-Aldrich (Cat No.81367–5G, America). Fibrin was provided by Thermo Scientific company (Cat No.MA126074, America). Ninety-eight percent formic acid was provided by Aladdin company (Cat No.F112034, China). Dulbecco’s modified eagle’s medium (DMEM), phosphate buffer saline (PBS, Cat No.P1022-500), fetal bovine serum (FBS, Cat No.S9030-200), CaCl₂ (Cat No.G0071) and lipase (Cat No.L8621) were from Solarbio (Beijing, China). Living cell staining kit (Cat No.C2015S), CCK-8 kit (Cat No.C0038), ELISA kit (Cat No.PI328), KCL (Cat No.ST1596) were purchased from Beyotime. Sodium nitroprusside (SNP, Cat No.S817931), adrenaline (AD, Cat No.N814761) and acetylcholine (Ach, Cat No.A886059) were obtained from Macklin (Shanghai, China). Anti-von Willebrand Factor antibody (ab115771), Anti-α-SMA (251411), Mouse Anti-CD68 antibody were obtained from Ruizekang Biotechnology Co., Ltd. (Beijing, China). Safranin O (Cat No.G1031), Verhoeff (Cat No. G1046) and Sirius red (Cat No.G1038) kit were supplied by Servicebio Biochemical Technology Co., Ltd (Wuhan, China). Mesenchymal stromal cells (MSCs) were obtained from Xinxiang Medical University (Henan, China). The use of MSCs has been approved by the Ethics Committee of Xinxiang Medical University.

Yang L., Li X., Wu Y., Du P., Sun L., Yu Z., Song S., Yin J., Ma X., Jing C., Zhao J., Chen H., Dong Y., Zhang Q, & Zhao L. (2020). Preparation of PU/Fibrin Vascular Scaffold with Good Biomechanical Properties and Evaluation of Its Performance in vitro and in vivo. International Journal of Nanomedicine, 15, 8697-8715.

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Combinatorial Enzymatic Hydrolysis Protocol

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Lipase (20 U/mg, operation temperature: 15–45 °C, operation pH: 4.0–11.0), cellulase (3 U/mg, operation temperature: 15–55 °C, operation pH: 3.5–6.0) and proteinase K (30 U/mg, operation temperature: 15–65 °C, operation pH: 4.0–12.5) were purchased from Solarbio Co. (Beijing, China). The cellulase was dissolved in the buffer solution at pH 4.8. Lipase and proteinase K were dissolved in sterile water. Combinatorial enzymes consisted of 22 U/mL Lipase, 3 U/mL cellulase and 45 U/mL proteinase K (final concentration) with a pH of 5.4.

Li Y., Dong R., Ma L., Qian Y, & Liu Z. (2022). Combined Anti-Biofilm Enzymes Strengthen the Eradicate Effect of Vibrio parahaemolyticus Biofilm: Mechanism on cpsA-J Expression and Application on Different Carriers. Foods, 11(9), 1305.

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Immobilized Probiotic Lactic Acid Bacteria

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OTA standard, 3-Mercaptopropyltriethoxysilane, N-hydroxysuccinimide, L-cysteine, thioglycolic acid, and microcrystalline cellulose, were purchased from Aladdin (Shanghai, China). L. plantarum PA01 was isolated from pickles and registered by the China Common Microbial Collections Management Center (CGMCC No.15660). All chromatographic pure reagents (acetonitrile, methyl alcohol, etc) were purchased from TEDIA (USA). Lipase, Folin-Ciocalteu, and Gallic acid were purchased from Solarbio (Beijing, China). All other annular-grade chemicals (glutaraldehyde, sodium hydroxide, hydrochloric acid, etc) were purchased from Sichuan Xilong Chemical Works (Sichuan, China). Grape juice was provided by Huiyuan Group (Beijing, China) and stored at −20 °C. Grape juice was thawed to room temperature before using.

Zhao M., Ren H., Yan Z., Ma J., Feng X., Liu D, & Long F. (2024). Reusable thiol-modification Lactobacillus plantarum embedded in cellulose nanocrystals composite aerogel for efficient removal of Ochratoxin A in grape juice. Food Chemistry: X, 22, 101336.

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Trace Element Analysis Protocol

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In our analyses, reagents were used as follows: H₂SO₄ (96%), HNO₃ (68%), HCl (37%), H₂O₂ (35%), HF (48%), NH₃·H₂O, methanol (HPLC quality), citric acid anhydrous (HPLC quality), and protease from Streptomyces griseus (type XIV, enzyme activity ≥ 3.5 units mg⁻¹) from Sigma-Aldrich. Sodium hydroxide (NaOH), NaBH₄, NH₃, Tris ((hydroxymethyl) aminomethane) were purchased from Sinopharm (Beijing). Driselase from Basidiomycetes sp. (protein ≥ 10%) and lipase (enzyme activity ≥20 units mg⁻¹) were supplied by Solarbio (Beijing). For the preparation of Se solutions, Na₂SeO₃ (Se(IV) 1000 mg L⁻¹), Na₂SeO₄ (Se(VI), 1000 mg L⁻¹) from Inorganic-Ventures (Christiansburg, VA, USA), Se-methionine (SeMet, >98.0%), Se-cystine (SeCys2, >98.0%), and selenomethylselenocysteine (MeSeCys, >98.0%) from TCI chemicals (Shanghai, China) were used.

Pinzon-Nuñez D.A., Wiche O., Bao Z., Xie S., Fan B., Zhang W., Tang M, & Tian H. (2023). Selenium Species and Fractions in the Rock–Soil–Plant Interface of Maize (Zea mays L.) Grown in a Natural Ultra-Rich Se Environment. International Journal of Environmental Research and Public Health, 20(5), 4032.

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Highland Barley Biochemical Characterization

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Highland barley was purchased from Xinlvkang Food Co., Ltd. (Qinghai, China). α-amylose (3700.0 U/g), pullulanase (1000.0 U/mL), and lipase (20,000.0 U/g) were obtained from Solarbio Science and Technology Co., Ltd. (Beijing, China). Isoamylase and pepsin (1:30,000.0) were acquired from Ruixiang Biological Technology (Shanghai, China) and Yuanye Bio-Technology (Shanghai, China), separately. Pancreatin (1:4000.0) was obtained from Ryon Biological Technology (Shanghai, China). Test kits for determining glucose, amylose, and protein were obtained from Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals and reagents were of analytical grade.

Wang C., Tian X., Zhang X., Zhang Z., Zhang X, & Zeng X. (2023). Physicochemical Characterizations, Digestibility, and Lipolysis Inhibitory Effects of Highland Barley Resistant Starches Prepared by Physical and Enzymatic Methods. Molecules, 28(3), 1065.

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Determination of Zearalenone and Metabolites

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Zearalenone (ZEN), α-zearalenol (α-ZOL), and β-zearalenol (β-ZOL) standards were purchased from Sigma-Aldrich (St. Louis, MO, USA) and stored at −20 °C. Stock solutions of ZEN (1 mg/L), α-ZOL (1 mg/L), and β-ZOL (1 mg/L) were prepared by dissolving them in 100% methanol. The standard solutions for high-performance liquid chromatography (HPLC) calibration were prepared by diluting the stock solution with a methanol/water (50/50, v/v) mixture. Other chemicals such as urea, sodium dodecyl sulfate (SDS), m-periodate, ploymyxin B, protease E, and lipase were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). The Lactobacillus biochemical identification Kit and De Man Rogosa and Sharpe (MRS) medium were provided by Huankai Microbial Science & Technology Co., Ltd. (Guangzhou, China). All the other chemicals used in this experiment were of chromatographic or analytical grade.

Gan M., Hu J., Wan K., Liu X., Chen P., Zeng R., Wang F, & Zhao Y. (2022). Isolation and Characterization of Lactobacillus paracasei 85 and Lactobacillus buchneri 93 to Absorb and Biotransform Zearalenone. Toxics, 10(11), 680.

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