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U plex mouse cytokine assay kit

Manufactured by Mesoscale
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The U-PLEX mouse cytokine assay kit is a multiplex assay system designed to detect and quantify multiple mouse cytokines simultaneously in a single sample. It provides a comprehensive and efficient way to measure various cytokine levels in mouse biological samples.

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U-PLEX assay (31 citations) U-PLEX kits (19 citations) U-PLEX (19 citations)

3 protocols using u plex mouse cytokine assay kit

1

Cytokine and Biomarker Quantification

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A portion of the colon was homogenized in PBS and cytokines were measured in 20 μL of the homogenate using U-PLEX mouse cytokine assay kit (Meso Scale Diagnostics, Rockville, MD) via the manufacturer's protocol. Mouse EIA kits from CUSABIO (CUSABIO Technology LLC, Houston, TX) were used to determine CgA (CSB-EL005344MO), CST (CSB-E17357m), and calprotectin (CSB-EQ013485MO).
Muntjewerff E.M., Tang K., Lutter L., Christoffersson G., Nicolasen M.J., Gao H., Katkar G.D., Das S., ter Beest M., Ying W., Ghosh P., Aidy S.E., Oldenburg B., van den Bogaart G, & Mahata S.K. (2021). Chromogranin A regulates gut permeability via the antagonistic actions of its proteolytic peptides. Acta physiologica (Oxford, England), 232(2), e13655.
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2

Measuring Cytokine Levels in BMDM Cells

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BMDM cells were preincubated with or without 100 μM TAT-NEMOActPep or MLN120B for 24 h. Negative control cells were treated with 1× PBS or dimethyl sulfoxide at the same concentrations as those present in either TAT-NEMOActPep or MLN120B, respectively. IL-1β and TNF-α levels were measured from 20 μl supernatant of cultured BMDM cells using U-PLEX mouse cytokine assay kit (Meso Scale Diagnostics) following the manufacturer’s protocol. The absorbance of samples was measured spectrophotometrically. Experiments were performed in triplicate.
Ko M.S., Cohen S.N., Polley S., Mahata S.K., Biswas T., Huxford T, & Ghosh G. (2022). Regulatory subunit NEMO promotes polyubiquitin-dependent induction of NF-κB through a targetable second interaction with upstream activator IKK2. The Journal of Biological Chemistry, 298(5), 101864.
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3

Plasma Cytokine Quantification

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Plasma (20 µl) cytokines were measured using U-PLEX mouse cytokine assay kit (Meso Scale Diagnostics, Rockville, MD) via the manufacturer’s protocol.
Liu M.A., Shahabi S., Jati S., Tang K., Gao H., Jin Z., Miller W., Meunier F.A., Ying W., van den Bogaart G., Ghosh G, & Mahata S.K. (2022). Gut microbial DNA and immune checkpoint gene Vsig4/CRIg are key antagonistic players in healthy aging and age-associated development of hypertension and diabetes. Frontiers in Endocrinology, 13, 1037465.
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