Total protein from kidneys and cells were extracted as previously described11 (link). Incubation was carried out with the following primary antibodies: anti-P2X7R (Santa Cruz Biotechnology, Dallas, TX, 1:200), anti-mouse NLRP3 (Adipogen, San Diego, CA, 1:1000), anti-human NLRP3 (Cell Signaling Technology, Beverly, MA, 1:500), anti-ASC (Santa Cruz Biotechnology, Dallas, TX, 1:200), anti-IL-1β (Abcam, Cambridge, MA, 1:500), anti-mouse capsase-1 (Adipogen, 1:1000), anti-human caspase-1 (Cell Signaling Technology, 1:500), anti-human cleaved caspase-1 (Cell Signaling Technology, 1:500, only for Supplementary Fig. 5c ), anti-ATP5a (Proteintech, Wuhan, China, 1:1000), anti-ATP5b (Proteintech, 1:1000), anti-Tubulin (Abcam, 1:2000), and anti-GAPDH (Cell Signaling Technology, 1:2000). After incubation with the appropriate horseradish peroxidase-conjugated IgG (Cell Signaling Technology), specific signals were determined on a Tanon 5200 Chemiluminescent Imaging System (Tanon, Shanghai) by using an ECL kit (Thermo Scientific).
Anti atp5b
Manufactured by Proteintech
Sourced in China
Anti-ATP5B is a primary antibody that targets the ATP5B subunit of the ATP synthase complex, which is a key enzyme involved in cellular energy production through the process of oxidative phosphorylation. This antibody can be used for various applications such as Western blotting, immunohistochemistry, and immunocytochemistry to detect and study the expression and localization of the ATP5B protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti tubulin, by Abcam (1 mentions)
Anti p2x7r, by Santa Cruz Biotechnology (1 mentions)
Anti atp5a, by Proteintech (1 mentions)
Anti human caspase 1, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated igg, by Cell Signaling Technology (1 mentions)
Ecl kit, by Thermo Fisher Scientific (1 mentions)
Anti il 1β, by Abcam (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti asc, by Santa Cruz Biotechnology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti tom20, by Proteintech (1 mentions)
Anti miro2, by Proteintech (1 mentions)
Anti miro1, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Anti gfp, by Thermo Fisher Scientific (1 mentions)
Anti myc, by Proteintech (1 mentions)
Anti β actin, by Proteintech (1 mentions)
Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
5 protocols using anti atp5b
1
Kidney and Cell Protein Extraction and Western Blot Analysis
2
Antibody Panel for Cellular Analyses
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The following antibodies were used in this study, anti‐ATP5B (ProteinTech/17247‐1‐AP), anti‐KIF5B (ProteinTech/21632‐1‐AP), anti‐Miro1 (Sigma/WH0055288M1), anti‐Miro2 (ProteinTech/11237‐1‐AP), anti‐HA (Abmart/M20003L), anti‐β‐tubulin (ProteinTech/10094‐1‐AP), anti‐β‐Actin (ProteinTech/60008‐1‐Ig), anti‐Tom20 (ProteinTech/11802‐1‐AP), anti‐Flag (Sigma/F3165), anti‐Myc (ProteinTech/60003‐2‐Ig), and anti‐GFP (Invitrogen/A6455). Alexa Fluor® 594 phalloidin was from Invitrogen (A12381). The polyclonal anti‐MICAL2PV antibody was generated by Sino Biological Inc. The secondary antibodies used for immunofluorescence, Alexa Fluor® 488 goat anti‐mouse (A21121), Alexa Fluor® 594 goat anti‐mouse (A21125), Alexa Fluor® 647 goat anti‐mouse (A31571), Alexa Fluor® 488 goat anti‐rabbit IgG (A11008), and Alexa Fluor® 594 goat anti‐rabbit IgG (A11012) were from Invitrogen. For Western blotting analyses, peroxidase‐linked donkey anti‐rabbit (NA934V) or peroxidase‐linked donkey anti‐mouse (NA931V) secondary antibodies from GE Healthcare were used.
3
Yeast Proteome Analysis: Protocols and Methods
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Yeast culture, UV cross-linking, cell lysis, 2C method and 2C-western blot experiments were done as previously described (Asencio et al. 2018 (link)). The following antibodies were used in western blot experiments: Anti-Pab1 1:4000 (Abcam, #ab189635), Anti-GAPDH 1:4000, a polyclonal antibody that detects all three Tdh (Tdh1, Tdh2, and Tdh3) yeast GAPDH isoforms (Sigma-Aldrich #G9545 [33]), Anti-histone H3 HRP 1:1000 (Abcam, #ab21054), Anti-Hexokinase 1:10000 (Bio-Rad, #4959-9988), Anti-Tpi 1:4000 (Proteintech, #10713-1-AP), Anti-tubulin 1:4000 (Abcam, #ab6160), and Anti-PFK antibody 1:4000 (Heinisch 1986 (link)). Peroxidase anti-peroxidase (PAP) antibody 1:10,000 was used to detect all TAP and Protein A tagged proteins (Sigma-Aldrich # P1291); Anti-ATP5b 1:1000 (Proteintech, #17247-1-AP) was used to detect the yeast ortholog protein ATP1.
4
Mitochondrial Protein Expression Analysis
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Total cell extracts or nuclear extracts were separated by SDS-PAGE and transferred to PVDF membranes. The following antibodies were used for immunoblot analysis: anti-HK2, anti-PFKM, anti-LDHa1, anti-PDK2, anti-PDHa1, anti-ATP5B, anti-IDH2, anti-HIF1α, anti-MnSOD, anti-Cu/ZnSOD, anti-Catalase, anti-GR, anti-GRX1, anti-GPX1, anti-PRX3 and anti-TRX2 antibodies and secondary antibody were purchased from Proteintech. anti-MnSOD (acetyl K68) antibody was purchased from Abcam. Anti-β-actin, anti-ComplexV, anti-Lc3B, anti-MFN1, and anti-Fis1 antibodies were from Cell Signaling Technology. Protein expression is visualized on Tanon-5200 Chemi-luminescent Imaging System (Tanon Science Technology).
5
Antibody Sources and Dilutions for Immunoblotting and Immunofluorescence
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Specific antibodies were purchased from the following commercial sources for the indicated experiments: anti-CS (ab129095, 1:2,000 for immunoblotting (IB)), anti-IMMT (ab137057, 1:1,000 or IB), anti-ANP (ab191398, 1:1,000 for IB, 1:50 for immunofluorescence), anti-BNP (ab92500, 1:10,00 for IB), anti-NFATC4 (ab62613, 1:1,000 for IB, 1:50 for IF), anti-MLC2v (ab92721, 1:50 for IF), goat anti-rabbit IgG H&L (Alexa Fluor 594) (150080, 1:250 for IF), goat anti-mouse IgG H&L (Alexa Fluor 488) (150113, 1:250 for IF) from Abcam. Additionally, anti-Tom20 (42406, 1:2,000 for IB, 1:50 for IF), anti-Vinculin (13901, 1:2,000 for IB), anti-OPA1 (67589S, 1:1,000 for IB, 1:50 for IP) from Cell Signaling Technology; anti-α-actinin (A7811, 1:200 for IF) were purchased from Sigma. From Abclonal, we purchased anti-ATP5B (A5769, 1:1,000 for IB), and anti-MLC2a (17283-1-AP, 1:50 for IF) from Proteintech. Anti-DYKDDDDK-tag (M20008, 1:10,000 for IB), anti-GAPDH (M20050, 1:5,000 for IB) were purchased from Abmart, and HRP goat anti-mouse IgG (H+L) (BK-R050, 1:5,000 for IB) and HRP goat anti-rabbit IgG (H+L) (BK-M050, 1:5,000 for IB) were purchased from Bioker.
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