Sds 19.1 software package

Manufactured by Thermo Fisher Scientific

The SDS 19.1 software package is a platform developed by Thermo Fisher Scientific for data analysis and reporting. It is designed to support the processing and interpretation of data generated by various analytical instruments and techniques.

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Lab products found in correlation

Qiaamp dna blood mini kit, by Qiagen (1 mentions) Abi 7500 sequence detection system, by Thermo Fisher Scientific (1 mentions) Taqman pcr detection, by Thermo Fisher Scientific (1 mentions)

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SDS software (178 citations) SDS 1.4 software (68 citations) SDS software package (5 citations)

2 protocols using sds 19.1 software package

Adenovirus Biodistribution in Orthotopic Xenograft

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Once the Hep3B orthotopic tumor were established, tumor-bearing mice were injected intravenously with 2.5 × 10¹⁰ VP of a2bm-d19 or Ha2bm-d19 three times every other day along with PBS as a negative control. The blood, muscle, lung, heart, kidney, spleen, liver, and tumor tissues were harvested at 72 h after the third injection of each virus, and DNA was extracted from the tissues using the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions^{57 (link)}. The number of Ad genomes was measured by Q-PCR (Applied Biosystems). Samples were analyzed in triplicates and data were processed by the SDS 19.1 software package (Applied Biosystems).

Yoon A.R., Hong J., Kim M, & Yun C.O. (2018). Hepatocellular carcinoma-targeting oncolytic adenovirus overcomes hypoxic tumor microenvironment and effectively disperses through both central and peripheral tumor regions. Scientific Reports, 8, 2233.

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Quantifying Adenoviral Genome Copy Number

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Human mesenchymal stromal cells were seeded in a 24-well plate at approximately 60~70% confluence. After 24 h, each cell was treated with naked oAd/RLX or oAd/RLX-PCDP complex at various MOIs for 18h. Then, the infected cells were washed twice with PBS and incubated with DMEM (5% FBS) for 4 and 8 days. Then, both supernatant and cells were collected, and the mixture underwent three cycles of freezing and thawing. The copy number of Ad genomes was analyzed by real-time quantitative PCR (Taqman PCR detection; Applied Biosystems, Foster City, CA) as previously described [40 (link)]. Samples were amplified for 40 cycles in an ABI 7500 sequence detection system (Applied Biosystems) and analyzed in triplicate and data were processed by the SDS 19.1 software package (Applied Biosystems).

Na Y., Nam J.P., Hong J., Oh E., Shin H.C., Kim H.S., Kim S.W, & Yun C.O. (2019). Systemic administration of human mesenchymal stromal cells infected with polymer-coated oncolytic adenovirus induces efficient pancreatic tumor homing and infiltration. Journal of controlled release : official journal of the Controlled Release Society, 305, 75-88.

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