Formvar and carbon coated 200 mesh copper grid

Manufactured by Ted Pella

The Formvar and carbon coated 200-mesh copper grid is a laboratory equipment item used for sample preparation in electron microscopy. It consists of a copper grid with a mesh size of 200 and is coated with a thin layer of Formvar, a polymer, and carbon. This grid provides a stable and uniform support for samples to be examined under an electron microscope.

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Lab products found in correlation

Gatan digital micrograph software, by Ametek (2 mentions) Orius sc200 ccd camera, by Ametek (2 mentions)

Close spelling variants of this product

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2 protocols using formvar and carbon coated 200 mesh copper grid

Ultrastructural Characterization of Tau Proteins

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Characterization of Tau-F, Tau-O and BDTau-F was performed by transmission electron microscopy (TEM) using negative staining. Tau-F were diluted 1:10 in distilled H2O and placed on a formvar and carbon coated 200-mesh copper grid (Ted Pella). The sample was directly stained with 2% uranyl acetate. Dried grids were examined by TEM (FEI Tecnaii G2) operated at 80 kV with an ORIUS SC200 CCD camera and Gatan Digital Micrograph software (both from Gatan Inc.).

Mothes T., Portal B., Konstantinidis E., Eltom K., Libard S., Streubel-Gallasch L., Ingelsson M., Rostami J., Lindskog M, & Erlandsson A. (2023). Astrocytic uptake of neuronal corpses promotes cell-to-cell spreading of tau pathology. Acta Neuropathologica Communications, 11, 97.

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Negative Staining TEM for Tau Fibrils

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Transmission electron microscopy (TEM) with negative staining was performed to confirm the presence of tau fibrils in tissue extracts. All samples were diluted 1:3 in distilled water and then transferred onto a formvar and carbon-coated 200-mesh copper grid (Ted Pella). Samples were stained with 2% uranyl acetate and were left to dry. Dried grids were examined using TEM (FEI Tecnaii G2) operated at 80 kV with an ORIUS SC200 CCD camera and Gatan Digital Micrograph software (Gatan Inc.).

Eltom K., Mothes T., Libard S., Ingelsson M, & Erlandsson A. (2024). Astrocytic accumulation of tau fibrils isolated from Alzheimer’s disease brains induces inflammation, cell-to-cell propagation and neuronal impairment. Acta Neuropathologica Communications, 12, 34.

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