Antibodies against fibronectin

Recombinant human transforming growth factor‐β1 (TGF‐β1) was purchased from Peprotech. Antibodies against fibronectin, P‐mTOR/mTOR, P62, LC3I/II were purchased from Cell Signalling Technology. Antibodies against α‐SMA, P‐PI3K/PI3K, P‐Akt/Akt, GAPDH were obtained from Affinity Biosciences. Goat pAbs against Rb IgG (HRP) and Rb pAbs against Ms IgG were obtained from ImmunoWay (China). TRIzol reagent was acquired from Ambion Life Technology (China). DEPC‐treated H2O and RNAse Away H₂O were purchased from Life Technologies. Fastking gDNA Dispelling RT SuperMix was purchased from Tiangen. UNICON® qPCR SYBR Green Master Mix and liposomal transfection reagents were purchased from Yevsen. The Masson's trichrome staining kit was obtained from Solarbio (China).

Antibodies: Antibodies against fibronectin and collagen I were purchased from Cell Signaling Technology (MA, USA). Antibodies against Sart1, Arginase 1, β-actin, Gapdh and F4/80 were purchased from Proteintech (Hubei, China). Recombinant IL-4 was purchased from BioLegend (CA, USA). Antibodies against STAT6, p-STAT6 and PPAR-γ were obtained from Cell Signaling Technology (MA, USA).
Lipids: Cholesterol and DSPC were acquired from Sigma-Aldrich, Inc. (St. Louis, MO). mPEG2000-DMG (MW2660) was purchased from NOF Co., Ltd. (Kawasaki Japan). The cationic lipidoid C12-200 was generated through ring opening of epoxides by amine substrates with a previously reported method 21 (link). Clodronate liposomes (anionic) were purchased from FormuMax (CA, USA).
Inhibitors: Inhibitors targeting STAT6 (AS1517499) and PPAR-γ (T0070907) were purchased from MedChemExpress (NJ, USA).
BLM was obtained from Hisun Pharmaceutical Co., Ltd (Zhejiang, China). An RT-PCR assay kit was supplied by Takara (Liaoning, China). A hydroxyproline assay kit was obtained from BioVision (CA, USA). Cell Counting Kit-8 was purchased from Dojindo Chemical technology Co. Ltd. (Shanghai, China).