Antibodies against stat3

High-purity grade (≥98%) caffeine and crystal violet were purchased from Aladdin Bio-Chem Technology, Co., Ltd. (Shanghai, China). NADP, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and a NADP/NADPH Quantification Kit were obtained from Sigma-Aldrich (St. Louis, MO, United States). D-Glucose 6-phosphate, recombinant G6PDH protein, and the G6PDH Activity Colorimetric Assay Kit were purchased from BioVision Incorporated (Milpitas, CA, United States). Reactive Oxygen Species Detection Reagents and DMEM were purchased from Thermo Fisher Biochemical Products (Beijing) Co., Ltd. FBS and a mixed P/S were purchased from Biological Industries (Kibbutz Beit Haemek, Israel) and Solarbio (Beijing, China), respectively. Primary antibodies against G6PDH, NOX4, NOX2, SOD2, and Cyclin D1 were obtained from Abcam (Cambridge, MA, United States). Antibodies against STAT3, p-STAT3, Ki67, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, United States). Anti-Catalase, anti-p47-phox, and anti-Cyclin E antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Anti-β-tubulin and horseradish peroxidase-conjugated secondary antibodies were purchased from Sino Biological, Inc. (Beijing, China) and R&D Systems (Minneapolis, MN, United States), respectively.

SFN was purchased from Sigma-Aldrich (St. Louis, MO, USA) and stock solution of SFN (100 mM) was dissolved in dimethyl sulfoxide (DMSO) and diluted to the appropriate concentration in cell culture medium with a maximal DMSO concentration less than 0.5%. Pyocyanin, NAC, and DAPI were purchased from Sigma-Aldrich. Cell Counting Kit-8 (CCK-8) kit was purchased from Dojindo Laboratories (Tokyo, Japan). IL-6 was obtained from R and D Systems (Minneapolis, MN, USA). Fetal bovine serum (FBS), modified Eagle’s medium (MEM), Dulbecco′s MEM (DMEM), penicillin, and streptomycin were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against STAT3, phospho-STAT3, JAK2, phospho-JAK2, Src, phospho-Src were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against cleaved caspase-3, Bcl-2, Bax and β-actin were purchased from Abcam (Cambridge, UK).

Fetal bovine serum (FBS), RPMI-1640 and Dulbecco's modified Eagle's medium (DMEM) were purchased from Gibco (Carlsbad, CA, USA). Gemcitabine was obtained from Eli Lilly (Indianapolis, IN, USA). Antibodies against STAT3, phospho-STAT3, E-cadherin and Snail were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against ALDH1A1, Ki67, and N-cadherin were purchased from Abcam (Cambridge, MA, USA). The antibody against SOCS5 was purchased from OriGene Technologies (Rockville, MD, USA). The anti-β-actin antibody used in this study was purchased from Medical & Biological Laboratories Co., Ltd. (Nagoya, Japan). Recombinant human IL-6 was obtained from PeproTech (Rocky Hill, NJ, USA), and AG490 was purchased from Selleckchem (Houston, TX, USA).