MCC was measured according to previous publications (39 (link), 40 ). Custom slides with 2-mm indentations were made to allow for whole trachea mounting. Mice were killed and tracheas were immediately removed, cut laterally to allow for the cilia to face the coverslip, and placed on the slide. Fifty microliters of FluoSpheres carboxylate, 0.2 μm crimson, 625/645 (Life Technologies) diluted 1:1,000 in PBS was placed on top of the trachea and mounted with coverslip. Within 5 min of trachea excision, the sample was imaged using an Opterra confocal microscope (Bruker) at 100 frames per second. All focus was matched to be ∼1 μm on top of the surface of the trachea, as flow rate decreases away from the surface of the tissue. Images were processed using ImageJ.
Opterra confocal microscope
Manufactured by Bruker
Sourced in United States
The Opterra confocal microscope is a high-performance imaging system designed for live-cell imaging and analysis. It utilizes a swept-field confocal technology to provide fast, high-resolution imaging with minimal phototoxicity. The Opterra system is capable of capturing real-time, high-speed, and high-contrast images of dynamic cellular processes.
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Lab products found in correlation
3 protocols using opterra confocal microscope
1
Tracheal Cilia Motility Imaging
2
Confocal Imaging of Bacterial Colonies
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Bacterial colonies were examined with a Bruker Opterra confocal microscope (Bruker, Billerica, MA, USA), imaging directly through the bulk gels. Image stacks were acquired with an 80 x 80 μm 2 field of view, 200 μm thick, sampling every 0.5 μm. 561 nm laser illumination was at 1-5% with an integration time of 100 ms. Embedded bacteria were imaged close to the coverglass and extending 200 μm up through the gel and into the cell culture medium. 3D volumes were projected into 2D using maximum intensity projections.
3
Confocal Imaging of Bacterial Colonies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacterial colonies were examined with a Bruker Opterra confocal microscope (Bruker, Billerica, MA, USA), imaging directly through the bulk gels. Image stacks were acquired with an 80 x 80 μm 2 field of view, 200 μm thick, sampling every 0.5 μm. 561 nm laser illumination was at 1-5% with an integration time of 100 ms. Embedded bacteria were imaged close to the coverglass and extending 200 μm up through the gel and into the cell culture medium. 3D volumes were projected into 2D using maximum intensity projections.
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