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T12 quick cryoem and cryoet

Manufactured by Thermo Fisher Scientific
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The T12 Quick CryoEM and CryoET is a laboratory instrument designed for cryo-electron microscopy and cryo-electron tomography applications. It provides rapid and efficient sample preparation for high-resolution structural analysis of biological specimens.

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Lab products found in correlation

Fcf400 cu, by Electron Microscopy Sciences (1 mentions) Zetapals, by Brookhaven Instruments (1 mentions)

4 protocols using t12 quick cryoem and cryoet

1

Cryo-EM Imaging of CDC-EV Samples

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CDC-EV samples were prepared for cryo-electron microscopy as previously described [25] (link) and imaged using a T12 Quick CryoEM and CryoET (FEI) at the UCLA Electron Imaging Center for NanoMachines (EICN) facility.
Gouin K., Peck K., Antes T., Johnson J.L., Li C., Vaturi S.D., Middleton R., de Couto G., Walravens A.S., Rodriguez-Borlado L., Smith R.R., Marbán L., Marbán E, & Ibrahim A.G. (2017). A comprehensive method for identification of suitable reference genes in extracellular vesicles. Journal of Extracellular Vesicles, 6(1), 1347019.
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2

Cryo-TEM Analysis of Hydrogel Proteins

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The hydrogel protein solutions (5 µl) were resuspended in gelation buffer and loaded onto a glow-discharged TEM grid (Electron Microscopy Sciences, FCF400-Cu) and stained with 0.8% uranyl formate. TEM images were obtained at 120 kV on a T12 quick cryoEM and cryoET (FEI).
Ying Y., Wang X.J., Vuong C.K., Lin C.H., Damianov A, & Black D.L. (2017). Splicing activation by Rbfox requires self-aggregation through its tyrosine-rich domain. Cell, 170(2), 312-323.e10.
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3

Insulin Complex Characterization

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Hydrodynamic size and zeta-potential of complexes were measured on a ZETAPALS (Brookhaven Instruments Corporation). The complexes were suspended in PBS with a final insulin concentration of 0.5 mg/mL. The zeta-potential of the complex at various glucose solutions was measured after adding glucose (0.4 g/mL) to complex suspension and incubating for 5 min. Of note, the particles were polydispersed and easy to precipitate, especially after the addition of glucose. Before observing the complex by SEM (ZEISS Supera 40VP) and TEM (T12 Quick CryoEM and CryoET (FEI)), the LL2-insulin complex was centrifuged, and PBS was replaced by deionized water. The concentration of complex was equivalent to 0.5 mg/mL insulin. The TEM sample was stained by phosphotungstic acid (2%).
Wang J., Wang Z., Chen G., Wang Y., Ci T., Hongjun L., Liu X., Zhou D., Kahkoska A.R., Zhou Z., Meng H., Buse J.B, & Gu Z. (2021). Injectable Biodegradable Polymeric Complex for Glucose-Responsive Insulin Delivery. ACS nano, 15(3), 4294-4304.
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4

Transmission Electron Microscopy of TTR Fibrils

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TEM was performed to visualize the fibril formation of TTR proteins and peptides. 5 μl of the sample was spotted onto freshly glow-discharged, carbon-coated EM grids (Ted Pella, Redding, CA). After 3 min of incubation, grids were rinsed three times with 5 μl of distilled water and then stained with 2% uranyl acetate for 2 min. Grids were examined with a T12 Quick CryoEM and CryoET (FEI) transmission electron microscope at an accelerating voltage of 120 kV. Digital images were recorded using a Gatan 2,048 × 2,048 charge-coupled device camera.
Saelices L., Johnson L.M., Liang W.Y., Sawaya M.R., Cascio D., Ruchala P., Whitelegge J., Jiang L., Riek R, & Eisenberg D.S. (2015). Uncovering the Mechanism of Aggregation of Human Transthyretin. The Journal of Biological Chemistry, 290(48), 28932-28943.
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