Phospho p70s6k pp70s6k

Manufactured by Cell Signaling Technology

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Phospho-p70S6K (pp70S6K) is a monoclonal antibody that detects the phosphorylated form of the p70 ribosomal protein S6 kinase (p70S6K). p70S6K is a serine/threonine protein kinase that plays a crucial role in the regulation of cell growth, proliferation, and protein synthesis in response to various extracellular signals.

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P70S6K (353 citations) Phospho-p70S6K (103 citations)

2 protocols using phospho p70s6k pp70s6k

Western Blot for Fibrotic Markers

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Proteins were loaded in equal amounts and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Merck Millipore). Membranes were incubated with the primary antibodies directed against the following molecules: α-SMA (Sigma-Aldrich) PDGF receptor-β (Cell Signaling Technology, Danvers, MA), collagen 1α1 (R&D Systems, Minneapolis, MN), JNK, phospho-JNK (pJNK), ERK1/2, phospho-ERK1/2 (pERK), SMAD2, phospho-SMAD2 (pSMAD2), AKT, phospho-AKT (pAKT), p70S6K, phospho-p70S6K (pp70S6K) (all from Cell Signaling Technology), GAPDH (Abcam, Cambridge, United Kingdom) and β-actin (Sigma-Aldrich). The following secondary antibodies were used as needed: a goat-anti-mouse-IgG-HRP antibody (Bio-Rad, Feldkirchen, Germany), a goat-anti-rabbit-IgG-HRP (Bio-Rad), or a goat-anti-sheep-IgG-HRP antibody (R&D Systems). Visualization was performed using the ChemoCam (INTAS, Homburg, Germany) after incubation with Clarity Western ECL Substrate (Bio-Rad).

Reiter F.P., Ye L., Ofner A., Schiergens T.S., Ziesch A., Brandl L., Ben Khaled N., Hohenester S., Wimmer R., Artmann R., He Y., Lee S.M., Mayr D., Zhang C., Gerbes A.L., Mayerle J., Denk G, & De Toni E.N. (2021). p70 Ribosomal Protein S6 Kinase Is a Checkpoint of Human Hepatic Stellate Cell Activation and Liver Fibrosis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 13(1), 95-112.

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Myogenesis Regulation by Signaling Pathways

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Rg1 (purity > 99%) was kindly provided by Professor Kim, Si-Kwan (Konkuk University, Chungju, Korea). Fetal bovine serum (FBS), horse serum (HS), and Dulbecco modified Eagle's medium (DMEM) were obtained from Gibco (Grand Island, NY, USA). Antibodies were as following: MHC (myosin heavy chain; the Developmental Studies Hybridoma Bank, Iowa, IA, USA), Akt, phospho-Akt (pAkt), p70S6K, phospho-p70S6K (pp70S6K), mTOR, phospho-mTOR (pmTOR), phospho-p38MAPK (pp38) (Cell Signaling Technology, Beverly, MA, USA), pan-Cadherin (Sigma, St. Louis, MO, USA), p38MAPK, MyoD, Myogenin, E2A, and α-Tubulin (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Go G.Y., Lee S.J., Jo A., Lee J., Seo D.W., Kang J.S., Kim S.K., Kim S.N., Kim Y.K, & Bae G.U. (2017). Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth. Journal of Ginseng Research, 41(4), 608-614.

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