We purchased luciferase reporter plasmids containing the 3′-UTRs of PRKCD and the corresponding mutated 3′-UTR (mouse). After that, we inserted the 3′-UTRs and mutated 3'-UTR into pRL-CMV reporter vector (Promega, USA). Then, 96-well plates were used for the incubation of 293T cells for 24 h. After that, a reporter plasmid and miR-181b mimics using Lipofectamine (Invitrogen) was co-transfected into each well. Subsequently, we detected renilla and firefly luciferase activities through Dual-Luciferase Reporter Assay System (E1910, Promega).
Prl cmv reporter vector
Manufactured by Promega
Sourced in United States
The PRL-CMV reporter vector is a plasmid-based system designed for gene expression studies. It contains a cytomegalovirus (CMV) promoter that drives the expression of the luciferase reporter gene, allowing for the quantification of transcriptional activity in various cell lines and experimental models.
Automatically generated - may contain errors
2 protocols using prl cmv reporter vector
1
Investigating miR-181b Regulation of PRKCD 3'UTR
2
Luciferase Assay for miRNA Target Validation
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We purchased luciferase reporter plasmids containing the 3'-UTRs of PRKCD and the corresponding mutated 3'-UTR. After that, we inserted the 3'-UTRs and mutated 3'-UTR into pRL-CMV reporter vector (Promega, USA). Then, 96-well plates were used for the incubation of 293T cells for 24h. After that, a reporter plasmid and miR-181b mimics using Lipofectamine 2000 (11668-019, Invitrogen) was cotransfected into each well. Subsequently, we detected renilla and re y luciferase activities through Dual-Luciferase Reporter Assay System (E1910, Promega).
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