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3 protocols using s butyrylthiocholine

1

Acetylcholinesterase Inhibition Assay

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Silica gel 60–120, Silica gel GF254, catechin, tricholoroacetic acid (TCA), donezepil, galantamine, and Tris-HCl were procured from Merck, Mumbai, India. Vincristine sulfate, dimethyl sulphoxide (DMSO), potassium ferricyanide, ferric chloride, and Folin-Ciocalteau reagent were from BDH chemicals Ltd., Poole, England. Acetylthiocholine and S-butyrylthiocholine and DTNB were purchased from Sigma-Aldrich, Darmstadt, Germany. Nutrient agar media was from Becton, Dickinson, and Company (Sparks, MD, USA). Petroleum ether and methanol were purchased from Duksan Pure Chemicals Company Limited (Ansan-si, Republic of Korea). The Institute of Nutrition and Food Science, University of Dhaka donated the cultures of bacteria and fungi. The other chemicals were of analytical grade.
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2

Antioxidant and Enzyme Inhibition Assays

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DPPH (1, 1′-diphenyl-2-picrylhydrazyl), aluminum chloride, ammonium molybdate, potassium ferricyanide, ascorbic acid, Folin-ciocalteu reagent, Tris–HCl and triton X-100 were obtained from Sigma-Aldrich, India. Gallic acid was obtained from Wako Pure Chemical Company Ltd., Japan. 2-deoxy-D-ribose, 2-thiobarbituric acid (TBA), catechin, quercetin, 5,5′-dithio-bis-(2-nitro) benzoic acid (DTNB), acetylthiocholine iodide, S-butyrylthiocholine, galantamine, and donepezil were obtained from Sigma-Aldrich, Japan. Unless otherwise specified, all other chemicals were of analytical grade.
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3

Screening of Phytochemical and Bioactive Potential

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2,2′-Diphenyl-1-picrylhydrazyl (DPPH), 5,5′-dithio-bis-(2-nitro) benzoic acid (DTNB), thiobarbituric acid (TBA), 2-deoxy-D-ribose, acetylthiocholine, S-butyrylthiocholine, donepezil, and galantamine were procured from Sigma-Aldrich, Germany. silica gel GF264, silica gel 60-120, Folin-Ciocalteu reagent, Tris-HCl, aluminum chloride, potassium ferricyanide, ammonium molybdate, trichloroacetic acid (TCA), and triton X-100 were from Merck, Darmstadt, Germany. Catechin, gallic acid, and ascorbic acid were purchased from Wako Pure Chemical Company Ltd., Osaka, Japan. Petroleum ether, chloroform, ethylacetate, and methanol were obtained from Active Fine Chemicals Limited, Dhaka, Bangladesh. Unless specified, all other chemicals were of analytical grade.
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