The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum samples were measured using the Sigma ALT and AST Activity Assay Kit (St. Louis, MO) and read using the BioTek Synergy H1 Hybrid Multi-Mode Reader plate reader (BioTek, Winooski, VT). Serum triglyceride content was measured using the Cayman Chemical Triglyceride Colorimetric Assay kit (Cayman Chemical, Ann Arbor, MI). Serum BDNF was measured using the R&D Systems (Minneapolis, MN, USA) Total BDNF Quantikine ELISA Kit and following the kit protocol and guidelines for serum tissue analysis.
Alt and ast activity assay kit
Manufactured by Agilent Technologies
The ALT and AST Activity Assay Kit is a laboratory tool used to measure the activity levels of the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in biological samples. The kit provides a colorimetric-based method to quantify the enzymatic activity of these analytes.
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2 protocols using alt and ast activity assay kit
1
Serum Biomarker Analysis Protocol
2
Serum Biomarker Quantification Protocol
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The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum samples were measured using the Sigma ALT and AST Activity Assay Kit (St. Louis, MO) and read using the BioTek Synergy H1 Hybrid Multi‐Mode Reader plate reader (BioTek, Winooski, VT). Serum triglyceride content was measured using the Cayman Chemical Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI). Serum BDNF was measured using the R&D Systems (Minneapolis, MN, USA) Total BDNF Quantikine ELISA Kit and following the kit protocol and guidelines for serum tissue analysis.
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