Or83b gal4

Manufactured by Bloomington Drosophila Stock Center

Or83b-GAL4 is a genetic tool used in Drosophila (fruit fly) research. It is a transgenic fly line that expresses the GAL4 transcriptional activator under the control of the Or83b odorant receptor promoter, allowing for the selective expression of genes of interest in a subset of olfactory receptor neurons.

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2 protocols using or83b gal4

Drosophila Rearing and Genetics Procedures

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Drosophila melanogaster flies were raised on standard medium (water, cornmeal, soy bean, agar, molasses, yeast, preservative methyl-4-hydroxybenzoate) at 25 °C, 60% humidity and 14 h/10 h light–dark cycles. Canton-S (CS) served as wild-type Drosophila strain in this study. The following colleagues kindly provided additional stocks: D. Armstrong (189Y-GAL4), M. Heisenberg (wild-type Canton-S, rut¹, and dnc¹), T.J. Hirsh (Trh493-GAL4), Y. Rao (5HT-1A-GAL4), H. Scholz (UAS-TNT), P. Shen (UAS-npf), H. Tanimoto (H24-GAL4, NP1131-GAL4 and mb247-GAL4; Tub-GAL80^ts), and T. Zars (mb247-GAL4). The following lines were obtained from the Bloomington Stock Center: Or83b-GAL4 (#2681), c305a-GAL4 (#30829), c739-GAL4(#7362), npf-GAL4 (#25681), 5HT-1B-GAL4 (#27637), UAS-NaChBac (#9469), UAS-5HT-1A^RNAi (#25834 and #33885), UAS-5HT-1B^RNAi (#27634 and #54006), UAS-CD4::tdTomato (#35837) and UAS-mCD8::GFP (#5130).

Ries A.S., Hermanns T., Poeck B, & Strauss R. (2017). Serotonin modulates a depression-like state in Drosophila responsive to lithium treatment. Nature Communications, 8, 15738.

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Drosophila Fly Stocks and Maintenance

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Oregon R (BL# 5), ninaB¹ (BL# 24776), Or67d-Gal4 (BL# 9998) and Or47b-Gal4 (BL# 9983) were obtained from Bloomington Drosophila Stock Center; UAS-hid was a gift from Dr John R. Nambu; Or83b-Gal4 was a gift from Dr Zuoren Wang and Or47b^+/+, Or47b^2/2 and Or47b^3/3 were generously provided by Dr Liming Wang. Flies were maintained on a standard corn flour, yeast and agar medium under a 12 L : 12 D cycle at 25°C. Naive male and virgin female flies were collected at eclosion. Males were kept individually for 3 days before behavioural tests.

Zhuang L., Sun Y., Hu M., Wu C., La X., Chen X., Feng Y., Wang X., Hu Y, & Xue L. (2016). Or47b plays a role in Drosophila males' preference for younger mates. Open Biology, 6(6), 160086.

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