Kidney lysates were prepared and Western blots performed as previously described19 (link). Quantification of Western Blots was by densitometry with analysis using Image J software20 (link). The following antibodies were used in the Western Blot analysis: anti-α-Smooth Muscle Actin—FITC antibody (Sigma-Aldrich, St. Louis, USA), anti-Fluorescein-POD Fab fragments (from goat Roche Applied Science, Indianapolis, USA), anti-GAPDH antibody (Rabbit monoclonal antibody, Cell Signaling technology, Massachusetts, USA), anti-Fibronectin antibody (Rabbit monoclonal antibody, Sigma-Aldrich, St.Louis, USA), anti-phospho-PFKFB2 (Ser485) (Rabbit monoclonal antibody, Cell Signaling Technology, Massachusetts, USA), anti-PFKFB2 antibody (Rabbit polyclonal antibody, abcam, Cambridge, UK), anti-PKM2 antibody (Rabbit monoclonal, Cell Signaling technology, Massachusetts, USA), anti-pPKM2 antibody (Rabbit polyclonal, Cell Signaling technology, Massachusetts, USA) and anti-rabbit Immunoglobulin HRP-linked (Swine polyclonal, Dako, Agilent Pathology Solutions, Santa Clara, USA).
Anti α smooth muscle actin fitc antibody
Manufactured by Merck Group
Sourced in United States
The Anti-α-Smooth Muscle Actin—FITC antibody is a fluorescent-labeled monoclonal antibody that specifically binds to α-smooth muscle actin, a cytoskeletal protein found in smooth muscle cells. This antibody is a useful tool for the identification and visualization of smooth muscle cells in various biological samples.
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2 protocols using anti α smooth muscle actin fitc antibody
1
Protein Expression Analysis in Kidney
2
Kidney Lysate and Western Blot Analysis
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Kidney lysate preparation and Western blot analysis was performed via standard methods as previously described [11 ]. Western Blots were analyzed for densitometry using Image J software. The following antibodies were used in the Western Blot analysis: anti-phospho-PFKFB2 Ser483 antibody (Rabbit monoclonal antibody, Cell Signalling Technology, Massachusetts, USA), anti-phospho-ACC Ser79 antibody (ABCAM, Cambridge, UK), anti-hexokinase-1 antibody (Rabbit antibody, Cell Signalling Technology, Massachusetts, USA), anti-PFK1M antibody (Rabbit, Sigma-Aldrich, St Louis, USA), anti-α-Smooth muscle actin–FITC antibody (Sigma Aldrich, St. Louis, USA), anti-Fibronectin antibody (Rabbit monoclonal antibody, Sigma-Aldrich, St Louis, USA), anti-PKM2 antibody (Rabbit monoclonal antibody, Cell Signalling technology, Massachusetts, USA), anti-rabbit Immunoglobulin HRP-linked antibody (Swine polyclonal, Dako, Agilent Pathology Solutions, Santa Clara, USA), anti-Fluorescein-POD Fab fragments (Goat, Roche Applied Science, Indianapolis, USA). Anti-GAPDH (Rabbit monoclonal antibody, Cell Signalling Technology, Massachusetts, USA) was used as a loading control.
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