Log In Sign up
The largest database of trusted experimental protocols

Anti nfix clone 3d2

Manufactured by Merck Group
Visit Supplier

Anti-NFIX (clone 3D2) is a laboratory reagent used for the detection and analysis of the nuclear factor I-X (NFIX) protein in biological samples. It is a monoclonal antibody that specifically binds to the NFIX protein, allowing researchers to study its expression and localization in cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Mes sds running buffer, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Santa Cruz Biotechnology (2 mentions) Anti ha, by Cell Signaling Technology (2 mentions) Anti bcl11a, by Abcam (2 mentions) Anti nfic, by Cell Signaling Technology (2 mentions) Anti nfia, by Active Motif (2 mentions) Anti lrf, by Thermo Fisher Scientific (2 mentions) Anti hemoglobin γ, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Anti-NFIX

2 protocols using anti nfix clone 3d2

1

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
+ Open protocol
+ Expand
2

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!