Light scattering was used to determine particle size and distribution at days 0 and 7 via a Malvern Mastersizer 2000, equipped with a wet cell Hydro 2000 SM dispersion unit (Malvern Instruments, Malvern, UK). The lyophilized emulsions were diluted with deionized water to produce light scattering obscuration values of 10–20%. An average value was determined by diluting 2 fresh samples per formulation and taking two readings per sample.
Hydro 2000 sm dispersion unit
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Hydro 2000 SM is a dispersion unit designed for the measurement of particle size distribution. It provides a controlled and reproducible way to disperse samples prior to analysis using laser diffraction or other particle sizing techniques.
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Lab products found in correlation
2 protocols using hydro 2000 sm dispersion unit
1
Particle Size Analysis of Lyophilized Emulsions
2
Thyme Oil Emulsion Preparation
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O/W emulsions were prepared by mechanical stirring. The emulsifier, 4.17 g of casein sodium salt (CAS: 9005-46-3, SIGMA-ALDRICH, Saint Louis, MO, USA), was dissolved in 100.14 g of water and stirred at 300 rpm for 2h. Then, 20.9 g of thyme essential oil (white, Food Chemicals Codex, CAS: 8007-46-3, SIGMA-ALDRICH) was added to the solution (water + casein) and homogenized at ambient temperature with an Ultra-Turrax (model T18, IKA, Staufern, Germany) at 15.000 and 20.000 rpm for 15, 30, 45 and 60 min to study the influence of homogenizer speed on the emulsion droplet-size distribution (Figure 1). The droplet-size distributions of the O/W emulsions were obtained by laser diffraction measurements using a Malver Mastersizer 2000 equipped with a Hydro 2000 SM dispersion unit (Malvern Instruments Ltd., Worcestershire, England).
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