Mouse anti-flag (M2) and mouse anti-β-actin were purchased from Sigma Aldrich. Mouse anti-ubiquitin was purchased from BD. Rabbit anti-flag, mouse anti-GFP, mouse anti-α-tubulin and rabbit anti-caveolin-1 antibodies were purchased from Cell Signaling. Rabbit anti-TfR antibody was a Proteintech Group product. The rabbit polyclonal antibodies for GM130, EEA1 and LAMP1 were all ABclone products. Rabbit anti-TGN46 was Abcam product, Fluor Alexa-488-conjugated goat anti-mouse IgG, Fluor Alexa-555-conjugated goat anti-mouse/rabbit IgG, and Alexa-647-conjugated goat anti-rabbit IgG were all purchased from Life Technologies. IRDye 680LT and 800CW goat anti-rabbit IgG or anti-mouse IgG antibodies were purchased from LI-COR Biosciences. The mouse monoclonal antibodies against K8, ORF45, ORF64, and ORF65, gH were generated in our lab previously.
Rabbit anti tgn46
Manufactured by Abcam
Rabbit anti-TGN46 is a primary antibody that recognizes the TGN46 protein. TGN46 is a trans-Golgi network membrane protein that serves as a marker for the trans-Golgi network. This antibody can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and study the localization of the TGN46 protein.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti gfp, by Cell Signaling Technology (1 mentions)
Rabbit anti caveolin 1, by Cell Signaling Technology (1 mentions)
Mouse anti ubiquitin, by BD (1 mentions)
Irdye 680lt, by LI COR (1 mentions)
Alexa 647 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Mouse anti α tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti flag, by Cell Signaling Technology (1 mentions)
Goat anti mouse igg fitc, by Merck Group (1 mentions)
Goat anti rabbit igg cy3, by Thermo Fisher Scientific (1 mentions)
Vectashield medium with dapi, by Vector Laboratories (1 mentions)
2 protocols using rabbit anti tgn46
1
Antibody Inventory for Western Blot
2
Immunostaining of GalNAc-T14 and TGN46
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The suspension of Dakiki cells containing 2 × 105 cells27 (link) was pelleted onto a microscopic slide using a CytoCentric 350 (Domel, Otoki, Slovenia). Cells were fixed using 4% paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% FBS in PBS for 1 hour, and stained with mouse anti-GalNAc-T14 mAb (1:100, Santa Cruz Biotechnology) and rabbit anti-TGN46 (marker of trans-Golgi network, 1:200, Abcam) antibodies in 10% FBS in PBS overnight at 4 °C. After washing, goat anti-mouse IgG-FITC (1:1,000, Sigma-Aldrich; Merck) and goat anti-rabbit IgG-Cy3 (1:2,000, Invitrogen) secondary antibodies in the 10% FBS in PBS were added and incubated for 1.5 hours at room temperature. Cover slips were mounted using Vectashield medium with DAPI (Vector Laboratories, Inc., Burlingame, CA) for fluorescence microscopy (Leica Microsystems GmbH, Wetzlar, Germany).
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