All fluorescent activated cell sorting (FACS) runs (sorting based on enhanced green fluorescence protein – EGFP levels) were performed in a BD FACSAria Cell Sorter fitted with a Blue Argon Laser 488 nm, violet diode 405 nm and red diode 633 nm (BD Biosciences, San Jose, CA, USA). After washing with 1 × PBS, cell pellets were resuspended in 1 × PBS each and then filtered through a 70-μm strainer. N/TERT and NHEK and SK-UT-1B wild-type cells were used for obtaining basal levels of green fluorescence (background autofluorescence), and all cells above this basal threshold were considered as being EGFP-positive fluorescent cells, and were thus sorted and plated in normal growth medium.
Blue argon laser 488 nm
Manufactured by BD
Sourced in United States
The Blue Argon Laser 488 nm is a compact and reliable laser instrument designed for various laboratory applications. The laser emits a blue wavelength of 488 nanometers, making it suitable for numerous scientific and research purposes. The device provides a stable and consistent output, ensuring reliable performance in the lab environment.
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2 protocols using blue argon laser 488 nm
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FACS Sorting of EGFP-Positive Cells
2
Cell Sorting and Transduction Protocol
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Transductions using pSIN-based constructs were carried out as previously described (Pantazi et al., 2014) .
Fluorescence-activated cell sorting FACS runs were performed in a BD FACSAria Cell Sorter fitted with a Blue Argon Laser 488 nm, violet diode 405 nm, and red diode 633 nm (BD Biosciences, San Jose, CA), as previously described (Pantazi et al., 2014) .
Fluorescence-activated cell sorting FACS runs were performed in a BD FACSAria Cell Sorter fitted with a Blue Argon Laser 488 nm, violet diode 405 nm, and red diode 633 nm (BD Biosciences, San Jose, CA), as previously described (Pantazi et al., 2014) .
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