Total histone h4

Manufactured by Cell Signaling Technology

Total histone H4 is a reagent used to detect and quantify the total amount of histone H4 protein in a sample. Histone H4 is a core component of nucleosomes, the fundamental unit of chromatin in eukaryotic cells. This product can be used to measure the overall levels of histone H4 in various biological samples.

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Histone H4 (19 citations)

2 protocols using total histone h4

Antibody Panel for Metabolic Enzyme Analysis

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Anti-USP13 antibody (1:2,000, A302-762A) was purchased from Bethyl Laboratories. Anti-OGDH antibody (1:1,000, 15212-1-AP) was purchased from Proteintech. Anti-ACLY (1:1,000, ab61762) antibody was purchased from Abcam. Anti-Ki-67 (1:200, D3B5), anti-AKT (1:500, #2920), anti-phospho-AKT (1:500, Ser73; #4060) and anti-PTEN (1:2,000, #9552) antibodies were purchased form Cell Signaling. Anti-actin (1:2,000, sc-1616), HRP-anti-goat IgG (1:3,000, #2020), HRP-anti-rabbit IgG (1:3,000, #2054) and HRP-anti-mouse IgG (1:3,000, #2055) antibodies were purchased from Santa Cruz. Antibodies against acetyl-histone H3 (Millipore #06-599), acetyl-histone H4 (1:1,000, Millipore #06-866), total histone H3 (1:1,000, Cell Signaling #9715) and total histone H4 (1:1,000, Cell Signaling #2592) were also used.

Han C., Yang L., Choi H.H., Baddour J., Achreja A., Liu Y., Li Y., Li J., Wan G., Huang C., Ji G., Zhang X., Nagrath D, & Lu X. (2016). Amplification of USP13 drives ovarian cancer metabolism. Nature Communications, 7, 13525.

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Western Blot Antibody Dilution Protocol

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All western blotting antibodies were diluted in TBST buffer with 5% bovine serum albumin (for antibodies targeting phosphorylated proteins) or 5% non-fat milk (for all other antibodies). The following antibodies were used for western blotting, diluted as recommended by the manufacturer; Total EGFR (Cell Signaling Technologies, cat. #: 4267), p-EGFR (Y1068) (Cell Signaling Technologies, cat. #: 2234), Total AKT (Cell Signaling Technologies, cat. #: 4685), p-AKT (S473) (Cell Signaling Technologies, cat. #: 4060), Total ERK1/2 (Cell Signaling Technologies, cat. #: 4695), p-ERK1/2 (T202/Y204) (Cell Signaling Technologies, cat. #: 4370), Total Histone H4 (Cell Signaling Technologies, cat. #: 13919), H4K12ac (Cell Signaling Technologies, cat. #: 13944) and Tubulin (Sigma Aldrich, cat. #: T5168).

Parag-Sharma K., Tasoulas J., Musicant A.M., Viesi do Nascimento-Filho C.H., Zhu Z., Twomey C., Liu P., Castilho R.M, & Amelio A.L. (2021). Synergistic Efficacy of Combined EGFR and HDAC Inhibitors Overcomes Tolerance to EGFR Monotherapy in Salivary Mucoepidermoid Carcinoma. Oral oncology, 115, 105166.

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