Goat anti sparc

Manufactured by R&D Systems

Goat anti-SPARC is an antibody that specifically binds to the SPARC (Secreted Protein Acidic and Rich in Cysteine) protein. SPARC is an extracellular matrix glycoprotein involved in various cellular processes. This antibody can be used in research applications to detect and study the SPARC protein.

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SPARC (8 citations)

3 protocols using goat anti sparc

Multicolor Immunofluorescence Staining of Tissues

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Tissues were fixed in Zn-formalin for 24 hours and embedded in paraffin for histological analysis and immunofluorescence staining. Sections were deparaffinized, rehydrated and prepared by antigen retrieval for 5 minutes each, and then blocked in 5% donkey serum for 1 hour at room temperature, incubated with primary antibodies for overnight at 4°C, washed, incubated with secondary antibodies for 1 hour at room temperature, washed and mounted. Primary antibodies used include goat anti-GFP (Abcam), rabbit anti-CD3 (Abcam), rat anti-EMCN (Santa Cruz), rabbit anti-β-catenin (Cell signaling), rabbit anti-Collagen type I (Abcam), rabbit anti-PDGF Receptor β (Abcam), rabbit anti-alpha-SMA (Abcam), goat anti-SPARC (R&D). Slides were imaged and visualized using an Olympus IX71 inverted multicolor fluorescent microscope and a DP71 camera.

Li J., Byrne K.T., Yan F., Yamazoe T., Chen Z., Baslan T., Richman L.P., Lin J., Sun Y.H., Rech A.J., Balli D., Hay C.A., Sela Y., Merrell A.J., Liudahl S.M., Gordon N., Norgard R.J., Yuan S., Yu S., Chao T., Ye S., Eisinger-Mathason T.S., Faryabi R.B., Tobias J.W., Lowe S., Coussens L.M., Wherry E.J., Vonderheide R.H, & Stanger B.Z. (2018). Tumor cell-intrinsic factors underlie heterogeneity of immune cell infiltration and response to immunotherapy. Immunity, 49(1), 178-193.e7.

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Immunofluorescence Analysis of Tissue Sections

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Immunofluorescence analyses were performed on formalin-fixed paraffin-embedded tissue sections as described^{76 (link)}. Antigen retrieval was accomplished by Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) at 99–100 °C for 20 min. Following retrieval, sections were stained with one or several of the following primary antibodies for immunodetection: mouse-anti-β-catenin (BD Transduction Laboratories, 610153), rabbit-anti-EGF (Abcam, ab9695), goat-anti-Smgc (Sigma, SAB2501988), rabbit-anti-Kal1 (aka Wfdc18; Abcam, Ab115270), rabbit-anti-Axin2 (Cell signalling, 2151), rabbit-anti-Wif1 (Abcam, ab186845), rabbit-anti-SMA (aka Acta2; Abcam, ab5694), goat-anti-Prol1 (Abcam, Ab119999), mouse-anti-PGRP (aka Pglyrp1; ThermoFisher, MA1-41044), rabbit-anti-Hepacam2 (Abcam, ab189943), goat-anti-Muc19 (Abcam, ab121014), mouse-anti-AQP5 (Santa Cruz Biotechnology, sc-514022), rabbit-anti-Clusterin (Abcam, ab92548), guinea pig-anti-CK8 (aka K8 or Krt8; Progen, GP-K8), rabbit-anti-CK14 antibodies (aka K14 or Krt14; ThermoFisher, MA5-11599), rabbit-anti-Klk1 (Boster, PA1709), mouse-anti-Ptn (Santa Cruz Biotechnology, SC74443), goat-anti-Sparc (R&D Systems; AF942). Secondary antibodies were conjugated with Cy2, Cy3, or Cy5 fluorochromes (Jackson ImmunoResearch Laboratories). Images were captured using an Axio imager Z1m and AxioCam MRm (Carl Zeiss) and a Leica TCS SP8.

Praktiknjo S.D., Obermayer B., Zhu Q., Fang L., Liu H., Quinn H., Stoeckius M., Kocks C., Birchmeier W, & Rajewsky N. (2020). Tracing tumorigenesis in a solid tumor model at single-cell resolution. Nature Communications, 11, 991.

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Immunohistochemical Analysis of Apcdd1 Mutants

Cited 1 time

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Immunohistochemistry on Apcdd1 mutants were as described previously (McKenzie et al., 2019 (link)). E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 μm and immunolabeled with Alexa488-tagged isolectin (1:100; Thermo Fisher Scientific); calbindin (1:1000; ImmunoStar). Sections were acquired as tiled maximum projection images for analysis using FIJI for blood vessel quantification and ImageJ plugin cell counting for segmentation and calbindin + cell counts. Western blot antibodies: Goat-anti-SPARC (1:1000; R&DSystems) and rabbit-anti-SerpinE1 (1:1000; Abcam) were used to detect EndoCM and HEK CM concentrated medium, both loaded with 20 μL. Xenograft 50 μm sections were immunolabeled with anti-Dlx2 (1:1000; Millipore).

Genestine M., Ambriz D., Crabtree G.W., Dummer P., Molotkova A., Quintero M., Mela A., Biswas S., Feng H., Zhang C., Canoll P., Hargus G., Agalliu D., Gogos J.A, & Au E. (2021). Vascular-derived SPARC and SerpinE1 regulate interneuron tangential migration and accelerate functional maturation of human stem cell-derived interneurons. eLife, 10, e56063.

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