Cd11b af594 clone m1 70

Manufactured by BioLegend

CD11b-AF594 (clone M1/70) is a fluorescently labeled anti-mouse CD11b antibody. CD11b is a cell surface integrin that is expressed on myeloid cells, including monocytes, macrophages, and neutrophils. This antibody conjugate can be used for the detection and analysis of CD11b-expressing cells in flow cytometry applications.

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Lab products found in correlation

Calprotectin, by Abcam (1 mentions) 4 hydroxynonenal antibody, by Abcam (1 mentions) Dneasy blood and tissue kit, by Qiagen (1 mentions) Liberase, by Roche (1 mentions) Anti cd16 32, by BioXCell (1 mentions) Bovine serum albumin (bsa), by BioXCell (1 mentions) Foxp3 fix perm kit, by BD (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Pimonidazole hydrochloride, by Hypoxyprobe (1 mentions)

Close spelling variants of this product

CD11b (clone M1/70, CD11b (M1/70, Clone M1/70 CD11b-AF594 M1/70 CD11b

2 protocols using cd11b af594 clone m1 70

Gastric Tissue Collection and Processing

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Stomach tissue collection was performed as described previously (10 (link)). Briefly, the stomachs were opened along the greater curvature. For histology, gastric strips from both the lesser and greater curvatures were fixed in formalin for paraffin sections. For DNA, gastric tissue was snap frozen in liquid nitrogen and extracted using the DNEasy Blood and Tissue Kit (Qiagen, Valencia, CA). For flow cytometry, gastric cells were digested using a modified version of the protocol described by Geem et al. (11 (link)), which utilizes 17.9 μg/ml Liberase TM (Cat #05401119001, Roche Diagnostics Corporation, Indianapolis, IN) instead of Type VIII collagenase. Immunohistochemistry and immunofluorescence were performed as described previously (10 (link)), using antibodies against the following targets: calprotectin (#ab22506, Abcam); CD11b-AF594 (clone M1/70, #101254, BioLegend); Cxcr2-AF488 (#FAB2164G, R&D Systems); GSII-FITC lectin (#FL-1211, Vector Labs, Burlingame); H⁺/K⁺-ATPase-β (#D032-3, Medical and Biological Laboratories, Woburn, MA); intrinsic factor (gift from David Alpers, Washington University, St. Louis, MO); E-cadherin-FITC (#612130, BD Biosciences, San Jose, CA); E-cadherin-AF647 (#560062, BD Biosciences); and 4 Hydroxynonenal antibody (#ab46545, Abcam).

Kao K.D., Grasberger H, & El-Zaatari M. (2023). The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology. Frontiers in Immunology, 14, 1147695.

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Hypoxia Mapping in Murine Tissues

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Mice were injected with pimonidazole hydrochloride in PBS (80mg/kg; Hypoxyprobe) intraperitoneally 1.5 hours prior to sacrifice. Tissues were dissected and processed as described above. For flow cytometry studies, pimonidazole was visualized using anti-pimonidazole antibodies (Pacific Blue Mab-1 clone 4.3.11.3; Hypoxyprobe) after cells were fixed for 20 min at 4°C using the FOXP3 Fix/Perm kit (BD Biosciences), and washed in permeabilization buffer. For imaging studies, dissected tumors were embedded in OCT and sectioned into 10μm cryosections. Cryosections were stored at −80°C until further use. For immunostaining, sections were fixed in 4% PFA (Electron Microscopy Sciences) for 20 minutes at RT, followed by a rinse in PBS containing 1% BSA (Sigma). Sections were blocked in 1% BSA in PBS containing anti-CD16/32 (BioXCell) for 1 hour at RT, and washed. Sections were stained with CD11b-AF594 (clone M1/70;BioLegend), CD31-AF647 (clone 390;BioLegend) and Pacific Blue Mab-1 (clone 4.3.11.3;Hypoxyprobe) for 1 hour at RT, followed by a wash and mounted using Vectashield (Vector Laboratories) and sealed with nail polish. Images were acquired on a Leica SP8 confocal microscope. Data analysis was performed using Imaris (Bitplane).

Kersten K., Hu K.H., Combes A.J., Samad B., Harwin T., Ray A., Rao A.A., Cai E., Marchuk K., Artichoker J., Courau T., Shi Q., Belk J., Satpathy A.T, & Krummel M.F. (2022). Spatiotemporal co-dependency between macrophages and exhausted CD8+ T cells in cancer. Cancer cell, 40(6), 624-638.e9.

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