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Nucleospin rna extraction kit

Manufactured by Thermo Fisher Scientific
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The NucleoSpin RNA extraction kit is a laboratory product designed for the isolation and purification of high-quality RNA from a variety of sample types. The kit utilizes silica-membrane technology to efficiently bind, wash, and elute RNA, ensuring reliable results for downstream applications.

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Lab products found in correlation

Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Rnalater, by Thermo Fisher Scientific (2 mentions) Applied biosystems 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions) Gotaq qpcr master mix, by Promega (1 mentions) Crimson taq dna polymerase, by New England Biolabs (1 mentions)

Close spelling variants of this product

RNA extraction kit (258 citations) NucleoSpin RNA (7 citations) NucleoSpin RNA kit (6 citations)

3 protocols using nucleospin rna extraction kit

1

Schistosoma mansoni and Trypanosoma brucei Protocols

Cited 1 time
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Schistosomula, juvenile and adult Schistosoma mansoni worms recovered 3, 4 and 6 weeks post infection of mice with 70 S. mansoni cercariae were obtained from the Schistosome Biology Supply Center (SBSC) of the Theodor Bilharz Research Institute (TBRI), stabilized using RNAlater (Invitrogen, ThermoFisher) and sent for processing from TBRI to the University of Glasgow. RNA was extracted from worms and schistosomula using a Macherey Nagel Nucleospin RNA extraction kit, and from this, cDNA was produced using Superscript III reverse transcriptase (ThermoFisher), all following manufacturer’s instructions. Genomic DNA was prepared from isolated worms at TBRI and shipped to University of Glasgow.
Bloodstream-form T. b. brucei Lister 427 2T1 strain [75 (link)] and its derivatives were maintained in HMI-11 medium [76 (link)], at 37°C in a 5% CO2 atmosphere, exactly as described [77 (link)].
Munday J.C., Kunz S., Kalejaiye T.D., Siderius M., Schroeder S., Paape D., Alghamdi A.H., Abbasi Z., Huang S.X., Donachie A.M., William S., Sabra A.N., Sterk G.J., Botros S.S., Brown D.G., Hoffman C.S., Leurs R, & de Koning H.P. (2020). Cloning and functional complementation of ten Schistosoma mansoni phosphodiesterases expressed in the mammalian host stages. PLoS Neglected Tropical Diseases, 14(7), e0008447.
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2

Quantifying Trypanosoma cruzi Life Stages

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Samples of T. cruzi Y strain epimastigotes, BTs and amastigotes collected from the supernatant of infected CC cultures were stabilized in RNAlater (Thermo Fisher). RNA was extracted using a Macherey-Nagel NucleoSpin RNA extraction kit and cDNA produced using Superscript III reverse transcriptase (Thermo Fisher). The expression profile of each of the five T. cruzi PDEs was generated from the three-stage cDNAs. GoTaq qPCR master mix (Promega) was used to amplify fragments, with an Applied Biosystems 7500 Real Time PCR System (primers in Table S2). The expression levels were calculated relative to the T. cruzi housekeeping gene GAPDH as a control (primers from Silber et al. 28 ).
De Araújo J.S., da Silva P.B., Batista M.M., Peres R.B., Cardoso-Santos C., Kalejaiye T.D., Munday J.C., De Heuvel E., Sterk G.J., Augustyns K., Salado I.G., Matheeussen A., De Esch I., De Koning H.P., Leurs R., Maes L, & Soeiro M.N.C. (2020). Evaluation of phthalazinone phosphodiesterase inhibitors with improved activity and selectivity against Trypanosoma cruzi. The Journal of antimicrobial chemotherapy, 75(4).
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3

Reverse Transcription and PCR of SUN and PP2A

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Total RNA was extracted from either 10-day-old seedlings or adult plant rosette leaves using a Nucleospin RNA extraction kit (ThermoFisher, http://www.thermofisher.com). Equal concentrations of total RNA were used as a template for cDNA synthesis, which was performed using a ProtoScript MuLV first-strand cDNA synthesis kit (NEB, https://www.neb.com). The coding sequences for AtSUN1, AtSUN2 and the reference gene Arabidopsis thaliana Protein Phosphatase 2A (Atpp2A) were amplified from cDNA using Crimson Taq DNA polymerase (NEB) and the following primers: 5 0 -AT-GTCGGCATCAACGGTGTCG-3 0 and 5 0 -TCGAGGTGAAGTCTAGCC-3 0 for AtSUN1, 5 0 -ATGTCGGCGTCAACGGTGTC-3 0 and 5 0 -TCAAGCAT GAGCAACAGAGAC-3 0 for AtSUN2, and 5 0 -ATGTCTATGGTTGATGA GCC-3 0 and 5 0 -GCTAGACATCATCACATTGTC-3 0 for PP2A. Equal volumes of cDNA were used as templates, and equal volumes of PCR reaction mix were loaded onto agarose gels for electrophoresis and analysis.
Varas J., Graumann K., Osman K., Pradillo M., Evans D.E., Santos J.L, & Armstrong S.J. (2015). Absence of SUN1 and SUN2 proteins in Arabidopsis thaliana leads to a delay in meiotic progression and defects in synapsis and recombination. The Plant journal : for cell and molecular biology, 81(2).
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