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Lkb1 sirna

Manufactured by GenePharma
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Sourced in China

LKB1 siRNA is a small interfering RNA that targets the LKB1 gene. It is designed to inhibit the expression of the LKB1 protein, which plays a role in cellular energy metabolism and tumor suppression.

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Lab products found in correlation

Ampk sirna, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 3000 rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Opti medium, by Thermo Fisher Scientific (1 mentions) Ampk sirna, by GenePharma (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions)

2 protocols using lkb1 sirna

1

Selective Knockdown of SIKs, AMPK, and LKB1

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Primary mouse hepatocytes were transfected with selective siRNA using Lipofectamine 3000 RNAiMAX Transfection Reagent (Thermo Fisher Scientific, Shanghai, China) for 24 or 48 h for follow-up experiments. The siRNA of SIKs was obtained from Life Technologies (Shanghai, China). AMPK siRNA was purchased from Santa Cruz (Shanghai, China). LKB1 siRNA was provided by GenePharma (Shanghai, China). Non-silencing RNA was purchased from GenePharma (Shanghai, China) and used as a negative control.
Liu S., Huang S., Wu X., Feng Y., Shen Y., Zhao Q.S, & Leng Y. (2020). Activation of SIK1 by phanginin A inhibits hepatic gluconeogenesis by increasing PDE4 activity and suppressing the cAMP signaling pathway. Molecular Metabolism, 41, 101045.
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2

Transfection and Treatment of HUVECs

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HUVECs cultured in collagen-coated 24-well plates were transfected with scrambled control siRNA, RhoA siRNA, AMPK siRNA or LKB1 siRNA (Genepharma, Shanghai, China) when cells reached 50% confluence. siRNA and RNA iMAX (Invitrogen, Camarillo, CA) were premixed in OPTI-medium (Invitrogen, Camarillo, CA) according to the manufacturer's instructions and then applied to cells. After 24 h transfection, OPTI-medium was replaced by complete HUVECs medium. Then HUVECs were treated with DPT for 30 min. All siRNA sense strands were listed in Table 1.
Wang Y., Wang B., Guerram M., Sun L., Shi W., Tian C., Zhu X., Jiang Z, & Zhang L. (2015). Deoxypodophyllotoxin suppresses tumor vasculature in HUVECs by promoting cytoskeleton remodeling through LKB1-AMPK dependent Rho A activation. Oncotarget, 6(30), 29497-29512.
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