Anti cd27

Manufactured by Miltenyi Biotec

Anti-CD27 is a monoclonal antibody that binds to the CD27 cell surface receptor. CD27 is a costimulatory molecule expressed on various lymphocyte subsets and plays a role in lymphocyte activation and proliferation. The Anti-CD27 product can be used for the detection and isolation of CD27-positive cells.

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Anti-CD27 APC (3 citations) Anti-CD27-APC-Vio770 (2 citations) Anti-CD27 (M-T271, (2 citations)

2 protocols using anti cd27

Immunophenotypic Characterization of B Cell Subsets and Recent Thymic Emigrants

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Immunophenotype was performed on heparinized peripheral blood samples obtained from each patient by means of flow cytometry. 100 μL of blood were stained with two multicolour antibody panels in order to evaluate different lymphocyte subsets, including B cell subpopulations (naïve follicular B cells, marginal zone B cells, switched memory B cells and transitional B cells) and recent thymic emigrants (RTE).
Panel for B cells analysis contained the following antibodies: anti-CD45, anti-CD19, anti-CD38 (Becton Dickinson), anti-CD27, anti-IgM, anti-IgG, anti-CD21, anti-CD10 and anti-IgD (Miltenyi Biotec).
Panel for RTE analysis contained the following antibodies: anti-CD45, anti-CD19, anti-CD4, anti-CD8 (Becton Dickinson), anti-CD3, anti-CD45RA, anti-CD31, anti-CD16 and anti-CD56 (Miltenyi Biotec).
Samples were acquired with MACSQuant Analyzer 10 (Miltenyi Biotec) and analyzed with FlowLogic software (version 7.2.1, Inivai Technologies).

Girardelli M., Valencic E., Moressa V., Margagliotta R., Tesser A., Pastore S., Spadola O., Athanasakis E., Severini G.M., Taddio A, & Tommasini A. (2021). Genetic and immunologic findings in children with recurrent aphthous stomatitis with systemic inflammation. Pediatric Rheumatology Online Journal, 19, 70.

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Profiling B Cell Subsets

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Flow cytometry was performed on a selection of samples from different stratified groups before and after stimulatory PBMC cultures. Cells were washed and underwent FcR blocking (Miltenyi Biotec) prior to staining with Zombie Aqua TM Fixable Viability Dye (BioLegend), anti-CD19 (BioLegend), anti-CD27 (Miltenyi Biotec), anti-CD38 (BioLegend) and anti-CD138 antibodies (BioLegend). Samples were analysed on the Attune NxT flow cytometer (ThermoFisher Scientific).

Moore T., Hossain R., Doores K.J., Shankar-Hari M, & Fear D.J. (2022). SARS-CoV-2-Specific Memory B Cell Responses Are Maintained After Recovery from Natural Infection and Postvaccination. Viral immunology, 35(6).

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