1 4 diamino 2 3 dicyano 1 4 bis 2 aminophenylthio butadiene u0126

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Sourced in Spain

1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126) is a chemical compound used in research applications. It functions as a potent and selective inhibitor of the extracellular signal-regulated kinase (ERK) pathway.

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Lab products found in correlation

Nerve growth factor (ngf), by BD (1 mentions) Pacap 38, by AnaSpec (1 mentions) 8 cpt camp, by Biolog (1 mentions) Ab130130, by Abcam (1 mentions) Ab105338, by Abcam (1 mentions) 3h 5 ht, by PerkinElmer (1 mentions) Ab13556, by Abcam (1 mentions)

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U0126 (99 citations)

2 protocols using 1 4 diamino 2 3 dicyano 1 4 bis 2 aminophenylthio butadiene u0126

Activating Neuronal Signaling Pathways

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Pituitary adenylate cyclase-activating polypeptide (PACAP-38) was purchased from AnaSpec. H-89 was purchased from Calbiochem and Nerve Growth Factor (NGF) from BD Biosciences. The cAMP analog 8-CPT-cAMP purchased from Biolog Life Science Institute. 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126) was obtained from Tocris. Drug stocks were prepared following manufacturers’ instructions, either in assay media or dimethylsulfoxide (DMSO). Final concentrations of DMSO in cell culture experiments did not exceed 0.1%, and vehicle control with matched DMSO concentration was used as appropriate.

Xu W., Dahlke S.P., Sung M., Samal B., Emery A.C., Elkahloun A, & Eiden L.E. (2022). Roles for ERK-dependent early- and late-gene expression in neuropeptide Gs-GPCR signaling for neuritogenesis. Journal of neuroendocrinology, 34(9), e13182.

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Modulation of Serotonin Signaling Pathways

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The drugs and substances used (abbreviations and respective suppliers in parentheses) were as follows: serotonin (5-hydroxytryptamine, 5-HT) from Sigma-Aldrich (St. Louis, MO, USA) and [ 3 H]-5-HT (specific activity 28 Ci/mmol) from Perkin-Elmer (Boston, MA, USA), γ-D-glutamylmeso-diaminopimelic acid (C-12-iE-DAP; DAP), lipopolysaccharide (LPS; Escherichiacoli
Kinase Inhibitor (gentamicin, receptor interacting protein-2 (RIP2) inhibitor) from InvivoGen (San Diego, CA, USA). 1,9-Pyrazoloanthrone (SP600125, c-Jun N-terminal kinases (JNK) inhibitor) and 1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)-butadiene (U0126, extracellular signalregulated kinase (ERK) inhibitor) were acquired from Tocris (Madrid, Spain). In addition, the specific primary antibodies: goat polyclonal antibody anti-human-SERT (ab130130), rabbit polyclonal antibody anti-human/mouse NOD1 (ab105338), rabbit polyclonal antibody antihuman TLR2 (ab24192) and rabbit polyclonal antibody anti-human TLR4 (ab13556) from Abcam

Layunta E., Latorre E., Forcén R., Grasa L., Plaza M.A., Arias M., Alcalde A.I, & Mesonero J.E. (2018). NOD1 downregulates intestinal serotonin transporter and interacts with other pattern recognition receptors. Journal of cellular physiology, 233(5).

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