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3 protocols using metaflumizone

1

Pesticide Bioassay Formulation Details

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Formulated chemicals used for bioassay were metaflumizone (130 g/L EC, BASF Corporation, Research Triangle Park, NC), indoxacarb (50 g/L EC, IPP, Guangdong Academy of Agricultural Sciences, Guangzhou, China). The oxidase inhibitor piperonyl butoxide (PBO, 95%) was purchased from Endura (Ravenna, Italy), the esterase inhibitor S,S,S-tributyl phosphorothioate (DEF, 98%) was from Sigma (St. Louis, MO), and the glutathine S-transferase depletor diethyl maleate (DEM, 95%) was from the Shanghai Chemical Reagent Co. Ltd. (Shanghai, China).
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2

Insecticide Compounds Sourcing and Characterization

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Metaflumizone (240 g/L SC) was obtained from the BASF Chemical Co., Ltd. (Shanghai, China). Indoxacarb (95%), abamectin (95%), beta-cypermethrin (96.1%), chlorfluazuron (95%), chlorfenapyr (95%), diafenthiuron (98%), and chlorantraniliprole (95%) were purchased from Hubei Kangbaotai Fine-Chemicals Co., Ltd. (Wuhan, China). Spinetoram (60 g/L SC) and spinosad (25 g/L SC) were purchased from the Dow AgroSciences Co., Ltd. (Shanghai, China). BT WG-001 (16000 IU/mg) was supplied by the Hubei Biopesticide Engineering Research Center. The following were obtained from the Sigma Chemical Co., Ltd. (St. Louis, MO, USA): triphenyl phosphate (TPP, reagent grade); diethyl maleate (DEM, reagent grade); piperonyl butoxide (PBO, reagent grade); 1-chloro-2,4-dinitrobenzene (CDNB); reduced glutathione (GSH); fast blue B salt; sodium-dodecyl sulphate (SDS); dithiothreitol (DTT); eserine; phenylmethanesulfonyl fluoride (PMSF). Alpha-naphthol acetate (α-NA) and ethylene diamine tetra-acetic acid (EDTA) were purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Roche Molecular Systems, Inc. (San Francisco, CA, USA). The protein-assay dye reagent was provided by Bio-Rad Laboratories, Inc. (Shanghai, China).
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3

Contact and feeding bioassays

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Chemical compounds used for contact bioassays were indoxacarb (Sigma-Aldrich, PubChem CID: 107720) and Metaflumizone (Sigma-Aldrich, PubChem CID: 11614934). The formulations used for feeding bioassays were Steward 30 WG (DuPont) for indoxacarb, and Alverde 24 SC (BASF) for Metaflumizone.
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