Anti ea d

Primary antibodies used in this study included anti-α-tubulin (Sigma-Aldrich, T6199), anti-acetyl-α-tubulin (Sigma-Aldrich, T6793), anti-ATG5 (Sigma-Aldrich, A0731), anti-β-actin (Merck Millipore, MAB1501), anti-BHRF1 (Merck Millipore, MAB8188), anti-c-Myc (Sigma-Aldrich, M4439), anti-Drp1 (Cell Signaling Technology, D6C7), anti-Ea-D (Santa Cruz Biotechnology, sc-58121), anti-GFP (Roche, 11814460001), anti-GFP (Santa Cruz Biotechnology, sc-9996), anti-HA (Santa Cruz Biotechnology, sc-9082 and sc-7392), anti-IRF3 (Novus Biologicals, SD2062), anti-LC3 (MBL International, PM036 and M151-3B), anti-pericentrin (Sigma-Aldrich, HPA016820), anti-TOM20 (BD Biosciences, 612278), anti-TOM20 (Cell Signaling Technology, D8T4N), anti-ZEBRA (Santa Cruz Biotechnology, sc-53904).
Secondary antibodies used in this study included Alexa Fluor 488 goat anti-rabbit, anti-mouse (Jackson ImmunoResearch, 111-545-003, 115-545-003), Alexa Fluor 555 goat anti-rabbit, anti-mouse (Life technology, A21428, A21424), Alexa Fluor 647 donkey anti-rabbit, anti-mouse (Life technology, A31573, A31571). Horseradish peroxidase (HRP)-labeled goat anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (115–035–003, 111–035–003).

Protein extracts were prepared using RIPA lysis buffer supplemented with protease inhibitor (Roche, Basel, Switzerland). The protein concentrations were determined using a protein assay reagent (Bio-Rad, Hercules, CA, USA) against a bovine serum albumin (BSA) standard curve. Equal amounts of proteins in each extract were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 0.45 µm nitrocellulose membranes. The blocked membranes were incubated with the appropriate primary antibodies. The primary antibodies used in this study include anti-BZLF1/Zta (BZ1, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000), anti-Rta (8C12, Argene, Varilhes, France;1:1000), anti-EA-D (1108-1, Santa Cruz Biotechnology; 1:1000); anti-BGLF4 (1:1000), VCAp18 (#PA1-73003, Invitrogen; 1:500); anti-cleaved caspase 3 (Asp175, Cell Signaling; 1:1000) and anti-Actin (13E5, Cell Signaling; 1:4000) antibodies. After being washed and incubated with secondary antibody. Signals in the blots were detected using the ChemiDoc Image system (Bio-Rad).