Coomassie brilliant blue g 250 protein stain powder

Ampicillin, isopropyl β-D-thiogalactopyranoside (IPTG), 40-nm gold nanoparticles (AuNPs), sodium phosphate monobasic, sodium chloride, imidazole, sucrose, Tween-20, and Whatman™ Grade 1 qualitative filter paper were purchased from Sigma—Aldrich (St. Louis, MO, USA). Halt protease inhibitor cocktail (100×), 10× phosphate buffered saline (PBS), HisPur nickel-nitrilotriacetic acid (Ni-NTA) resin, and BD Difco Luria-Bertani (LB) Broth were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Coomassie Brilliant Blue G-250 protein stain powder, Precision Plus Protein All Blue Prestained Protein Standards, and Protein Assay Dye Reagent Concentrate were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Bovine serum albumin (BSA) and dithiothreitol (DTT) were purchased from VWR Life Science (Radnor, PA, USA) and LPS Solution (Daejeon, Korea), respectively. A 30% acrylamide-bis solution was purchased from BiOSESANG (Seongnam, Korea). SARS-CoV-2 (2019-nCoV) spike antibodies, chimeric MAb (40150-D006), SARS-CoV-2 spike neutralizing antibody, mouse MAb (40591-MM43), and SARS-CoV spike/S1 protein were purchased from Sino Biological (Wayne, PA, USA). The receptor-binding domain (RBD) of the SARS-CoV-2 spike S1 protein was purchased from HyTest (Turku, Finland). Cultured SARS-CoV-2 was purchased from ATCC (Manassas, VA, USA).