MDA-MB-231 cells (ATCC HTB-26) were cultured in an incubator maintained at 37°C with 5% CO2 under full growth medium consisting of Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% Donor Bovine Serum (Gibco), 1% Penicillin-Streptomycin (Gibco), 1% Non-Essential Amino Acids (Gibco), and 1% L-glutamine (Gibco). MCF-10A cells were cultured in full growth medium consisting of DMEM/F12 (Gibco) with 5% Donor Horse Serum, 20 ng/mL epidermal growth factor (Corning), 10 μg/mL insulin, 0.5 μg/mL hydrocortisone, 100 ng/mL cholera toxin, and 1% Penicillin-Streptomycin. Transfections of GFP-Lamin A, GFP-H1.1 or RFP-lifeact (iBidi) were performed with Lipofectamine 3000 (ThermoFisher Scientific) in OptiMEM serum-free media (ThermoFisher) following the manufacturer’s suggested protocols. Transduction of Ad-NLS-GFP (a gift from Dr. Daniel Conway, originally from Vector Biosystems INC.) were performed with polybrene at final concentration of 5 μg/ml in normal culture medium.
Donor horse serum
Manufactured by Corning
Sourced in United States
Donor horse serum is a type of lab equipment used as a cell culture supplement. It provides essential nutrients, growth factors, and other components needed to support the growth and maintenance of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Corning (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Opti mem serum free media, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Corning (1 mentions)
Donor bovine serum, by Thermo Fisher Scientific (1 mentions)
Recombinant human il 2, by Thermo Fisher Scientific (1 mentions)
Nk 92, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Celltracker cmtmr, by Thermo Fisher Scientific (1 mentions)
3 protocols using donor horse serum
1
Culturing and Transfecting Breast Cell Lines
2
Cell Culture Protocols for Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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T-47D, NK-92, 293 and HeLa cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured following the methods recommended by ATCC at 37°C in a 5% CO2 humidified incubator. All the media, fetal bovine serum and donor horse serum were purchased from Corning (NY, USA); other supplements were from ThermoFisher Scientific (MA, USA). Two hundred units per ml of recombinant human IL-2 from PeproTech (NJ, USA, Cat. 200–02) was used in NK-92 culture.
3
Adhesion of C2C12 Myoblasts to Actuating Hydrogels
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C2C12 myoblasts were maintained in culture
at 37 °C with 5% CO2 concentration in growth media
(DMEM (Gibco) supplemented with 10% fetal bovine serum (Corning) and
1% penicillin–streptomycin (Corning)), and passaged no more
than 15 times before use in experiments. Glass slides with hydrogels
were fitted into metal imaging chambers and cells were plated at a
density of 60,000 cells/chamber in growth media and allowed to attach
overnight before experiments were conducted. To validate cell adhesion
to actuating hydrogels, gels were formed with and without laminin,
and adhered cells were visualized with 10 μM CellTracker CMTMR
(Invitrogen). The number of attached cells and cell spreading area
were quantified.
For experiments longer than 1 day, cell media
was changed daily, using growth media until cells reached ∼80%
confluency, at which point media was changed to DMEM supplemented
with 2% donor horse serum (Corning) and 1% penicillin–streptomycin.
at 37 °C with 5% CO2 concentration in growth media
(DMEM (Gibco) supplemented with 10% fetal bovine serum (Corning) and
1% penicillin–streptomycin (Corning)), and passaged no more
than 15 times before use in experiments. Glass slides with hydrogels
were fitted into metal imaging chambers and cells were plated at a
density of 60,000 cells/chamber in growth media and allowed to attach
overnight before experiments were conducted. To validate cell adhesion
to actuating hydrogels, gels were formed with and without laminin,
and adhered cells were visualized with 10 μM CellTracker CMTMR
(Invitrogen). The number of attached cells and cell spreading area
were quantified.
For experiments longer than 1 day, cell media
was changed daily, using growth media until cells reached ∼80%
confluency, at which point media was changed to DMEM supplemented
with 2% donor horse serum (Corning) and 1% penicillin–streptomycin.
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