Urea, dithiothreitol (DTT), iodoacetamide (IAA), iron chloride, triethylammonium bicarbonate (TEABC), ethylenediaminetetraacetic acid (EDTA), ammonia phosphate (NH4H2PO4), trifluoroacetic acid (TFA), formic acid (FA), acetonitrile (ACN), n-Dodecyl β-D-maltoside (DDM), protease, phosphatase inhibitor, DMSO (HPLC grade), and Phosphate-Buffered Saline (PBS) were obtained from Sigma (St. Louis, MO). The Ni-NTA silica beads and agarose beads were obtained from Qiagen (Hilden, Germany) and the EmporeTM extraction C18 disks were obtained from 3 M (St. Paul, MN). TMT-16 reagents were purchased from Thermo Fisher Scientific (Waltham, MA). Water was processed using a Millipore Milli-Q system (Bedford, MA). Polypropylene microwell chip with 2.2-mm wells diameter was manufactured on polypropylene substrates by Protolabs (Maple Plain, MN). Tris(2-carboxyethyl) phosphine hydrochloride (TCEP-HCl), and 50% Hydroxylamine (HA) were purchased from Thermo Fisher Scientific (Waltham, MA). Ethanol was purchased from Decon Labs, Inc. (King of Prussia, PA).
Ni nta silica beads
Manufactured by Qiagen
Ni-NTA silica beads are a type of solid-phase chromatography resin used for the purification of recombinant proteins. The beads are made of silica and have a nickel-nitrilotriacetic acid (Ni-NTA) coating, which allows for the selective binding of proteins with a histidine-tag. This property enables the efficient capture and recovery of target proteins from complex mixtures.
Automatically generated - may contain errors
Lab products found in correlation
Milli q system, by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
Phosphatase inhibitor, by Merck Group (1 mentions)
Hydroxylamine ha, by Thermo Fisher Scientific (1 mentions)
Tmt 16 reagents, by Thermo Fisher Scientific (1 mentions)
Dmso hplc grade, by Merck Group (1 mentions)
Agarose beads, by Qiagen (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride tcep hcl, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Urea, by Bio-Rad (1 mentions)
Triethylammonium bicarbonate, by Merck Group (1 mentions)
Sequant zic hilic column, by Merck Group (1 mentions)
S trap microcolumns, by Protifi (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride, by Merck Group (1 mentions)
Sequencing grade modified trypsin, by Promega (1 mentions)
2 protocols using ni nta silica beads
1
Quantitative Proteomics Sample Preparation
2
Protein Sample Preparation for MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Urea was purchased from Bio-Rad (Hercules, CA). Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), 2-chloroacetamide (CAA), triethylammonium bicarbonate (TEAB), iron chloride, trifluoroacetic acid (TFA), ethylenediaminetetraacetic acid (EDTA), and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich. Phosphoric acid (PA) was purchased from Honeywell Fluka. Methanol (MeOH), formic acid (FA) and SeQuant ZIC-HILIC column were purchased from Merck. Acetic acid (AA), acetonitrile (ACN), and ammonia phosphate (NH4H2PO4) were purchased from J.T.Baker. Ni-NTA silica beads were purchased from Qiagen. Evotips were purchased from Evosep. S-Trap micro columns were purchased from ProtiFi (Huntington, NY). Empore C8 extraction disks were purchased from 3M. MS grade Lys-C (lysyl endopeptidase) was purchased from FUJIFILM Wako. Sequencing-grade modified trypsin was purchased from Promega. Water was obtained from a Millipore Milli-Q system.
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