Cd11b bv605 m1 70

Manufactured by BD

Sourced in United States

CD11b BV605 (M1/70) is a fluorescently-labeled antibody that binds to the CD11b protein, which is expressed on the surface of various immune cells such as monocytes, macrophages, and neutrophils. This product can be used in flow cytometry applications to identify and quantify CD11b-positive cells.

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Lab products found in correlation

Lsrfortessa, by BD (1 mentions) Streptavidin af488, by Thermo Fisher Scientific (1 mentions) Cd19 pe 1d3, by BD (1 mentions) B220 apc ef780, by Thermo Fisher Scientific (1 mentions) Fc block anti cd16 32, by BD (1 mentions) B220 percp, by BD (1 mentions) Cd19 fitc 1d3, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Efluor 506, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD11b (454 citations) CD11b (M1/70, (58 citations) M1/70 (41 citations) CD11b-BV605 (6 citations)

2 protocols using cd11b bv605 m1 70

Multicolor Flow Cytometry Panel

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Cells were incubated with Fc block (anti-CD16/32, BD) and stained with different panels of fluorochrome-conjugated monoclonal antibodies in PBS/2% FBS using the following antibodies: CD5 BV421 (S3-7.3), CD19 PE (1D3), CD19 FITC (1D3), CD19 BUV395 (1D3), CD43 APC (S7), B220 AF-700 (RA3-6B2), B220 PerCP, CD11b BV605 (M1/70) (all BD), CD93 APC (AA4.1), CD93 PE (AA4.1), B220 APC-EF780 (RA3-6B2), IgM eFlour450 (II/41) (all eBioscience), CD5 biotin (Biolegend), and IgM FITC (Fab₂, polyclonal) (Southern Biotech). In some antibody panels, primary staining was followed by addition of streptavidin-AF488 (Life technologies). Samples were run using a BD LSRFortessa or LSR II and data were analyzed in Flowjo v9.6.4 (Treestar).

Pedersen G.K., Li X., Khoenkhoen S., Ádori M., Beutler B, & Karlsson Hedestam G.B. (2018). B-1a Cell Development in Splenectomized Neonatal Mice. Frontiers in Immunology, 9, 1738.

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Comprehensive Immune Cell Analysis

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The following antibodies/viability dyes were used: CD11b-PE/Cy7 (M1/70), CD45-BV605 (30F-11), Ly6C-BV711 (HK1.4), CD11c-APC/Cy7 (N418), Ly6G-PerCP/Cy5.5 (1A8), MHCII-AF700 (M5/114.15.2), CD64-APC (X54-5/7.1), CD3-BV785 (17A2), CD4-APC (GK1.5), CD8-PerCP (YTS156.7.7), NK1.1-BV650 (PK136) from Biolegend; CD45-AF700 (30F-11), CD24-PE (M1/69), CD103-FITC (2E7), fixable viability dye eFluor506 and eFluor780 from eBioscience; CD144 (VE-cadherin, 11D4.1), SiglecF-BV421 (E50-2440), CD11b-BV605 (M1/70) from BD Biosciences; donkey anti-rat IgG (H+L)-AF488 from Jackson; propidium iodide (PI), beta-actin (AC-15) (Sigma-Aldrich); anti-cIAP1 (1E1-1-10; Enzo Life Sciences or HAP002317 (Atlas Antibodies) using wildtype, ciap1^−/−, ciap2^−/− fibroblasts as controls for antibodies; goat anti-rat IgG (H+L)-HRP from ENZO Life sciences (ADI-SAB-220-0500), donkey anti-mouse and anti-rabbit IgG (H+L)-HRP from SouthernBiotech (Birmingham, AL, USA).

Vasilikos L., Hänggi K., Spilgies L.M., Kisele S., Rufli S, & Wong W.W. (2021). Loss of cIAP1 in Endothelial Cells Limits Metastatic Extravasation through Tumor-Derived Lymphotoxin Alpha. Cancers, 13(4), 599.

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