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Flare comet slides

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Flare comet slides are specialized microscope slides used for the comet assay, a technique to measure DNA damage and repair in individual cells. The slides are coated with agarose gel and designed to facilitate the visualization and analysis of DNA migration patterns, which are indicative of DNA fragmentation or strand breaks.

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Lab products found in correlation

Lmagarose, by Bio-Techne (3 mentions) Fluorescence microscope, by Olympus (2 mentions) Bx41 fluorescent microscope, by Olympus (1 mentions)

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FLARE™ slides (3 citations)

3 protocols using flare comet slides

1

Alkaline Comet Assay for DNA Damage

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Alkaline comet assay was performed to determine DNA damage induced by BA/A. 3,000–5,000 cells were collected after appropriate treatment, mixed with LM Agarose (Trevigen, Gaithersburg, MD) and allowed to solidify on flare comet slides (Trevigen) at 4 °C. The slides were then immersed in lysis buffer (10 mM Tris, pH 10, 2.5 M NaCl, 100 mM EDTA, 1% Triton X-100, 10% DMSO) for 2 h at 4 °C. Electrophoresis was then performed on slides in alkaline conditions (300 mM NaOH, 1 mM EDTA, pH > 13). The slides were then washed, fixed with 100% cold ethanol, stained with ethidium bromide, and visualized with Olympus BX41 fluorescent microscope (Olympus, Pittsburg, PA). Tail Moment was measure in a minimum of 50–70 cells per treatment using the Open Comet software.
Bhardwaj V., Gokulan R.C., Horvat A., Yermalitskaya L., Korolkova O., Washington K.M., El-Rifai W., Dikalov S.I, & Zaika A.I. (2017). Activation of NADPH oxidases leads to DNA damage in esophageal cells. Scientific Reports, 7, 9956.
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2

Alkaline Comet Assay for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine levels of DNA damage, comet assay was performed in alkaline conditions. Briefly, 3000–5000 cells were mixed with LM Agarose (Trevigen, Gaithersburg, MD) and allowed to solidify on flare comet slides (Trevigen, Gaithersburg, MD) at 4°C. Then, the slides were incubated in lysis buffer for 2 hours at 4°C. Electrophoresis was performed on slides in alkaline conditions. After washing, the slides were fixed with 100% cold ethanol, stained with ethidium bromide, and visualized with fluorescence microscope (Olympus, Pittsburg, PA). A minimum of 50–70 cells were analyzed using the Open Comet software.
Gokulan R.C., Paulrasu K., Azfar J., El-Rifai W., Que J., Boutaud O.G., Ban Y., Gao Z., Buitrago M.G., Dikalov S.I, & Zaika A.I. (2023). Protein adduction causes non-mutational inhibition of p53 tumor suppressor. Cell reports, 42(1), 112024.
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3

Alkaline Comet Assay for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine levels of DNA damage, comet assay was performed in alkaline conditions. Briefly, 3000–5000 cells were mixed with LM Agarose (Trevigen, Gaithersburg, MD) and allowed to solidify on flare comet slides (Trevigen, Gaithersburg, MD) at 4°C. Then, the slides were incubated in lysis buffer for 2 hours at 4°C. Electrophoresis was performed on slides in alkaline conditions. After washing, the slides were fixed with 100% cold ethanol, stained with ethidium bromide, and visualized with fluorescence microscope (Olympus, Pittsburg, PA). A minimum of 50–70 cells were analyzed using the Open Comet software.
Gokulan R.C., Paulrasu K., Azfar J., El-Rifai W., Que J., Boutaud O.G., Ban Y., Gao Z., Buitrago M.G., Dikalov S.I, & Zaika A.I. (2023). Protein adduction causes non-mutational inhibition of p53 tumor suppressor. Cell reports, 42(1), 112024.
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