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2 protocols using anti vinculin e1e9v 13 901

1

Antibody Panel for Signaling Pathway Analysis

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The following antibodies were utilized per manufacturers’ recommendations: rabbit monoclonal anti-phospho-Stat3 (Tyr705, D3A7, 9145), anti-Stat3 (79D7, 4904), anti-PARP (46D11), anti-Caspase-3 (9662), anti-cleaved Caspase 3 (Asp175, 5A1E, 9664), anti-Tyro3 (D38C6, 5585), anti-phospho-Akt (Ser473, D9E, 4060), anti-PDGFRβ (28E1, 3169), anti-Akt (11E7, 4685), anti-cyclin D1 (92G2, 2978), and anti-Vinculin (E1E9V, 13,901) from Cell Signaling (Danvers, MA); rabbit monoclonal anti-PDGF Receptor beta (Y92, ab32570) from Abcam (Cambridge, MA); mouse monoclonal β-actin (A1978), and anti-phospho-Erk 1/2 (AW39R) from Sigma Aldrich (Millipore Sigma, St. Louis, MO). Crizotinib was purchased from Sigma Aldrich and LC Laboratories (Woburn, MA) and sunitinib was purchased from Selleckchem (Houston, TX).
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2

Western Blot Antibodies for STRAP

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Trypan blue stain was obtained from Life Technologies Corporation (Grand Island, NY, USA). Primary antibodies used for Western blotting included: polyclonal rabbit anti-STRAP (18277–1-AP) from Proteintech (Rosemont, IL, USA) and monoclonal mouse anti-β-actin (A1978) and anti-FLAG (F1804) from Sigma Aldrich (St. Louis, MO). The anti-phospho-FAK (Tyr397, D20B1, #8556), anti-FAK [(D5O7U) XP® (#71433)], and anti-vinculin (E1E9V, #13901) rabbit monoclonal antibodies were from Cell Signaling (Danvers, MA). Antibodies were used according to manufacturers’ suggestions.
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