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2 protocols using sds page gel rapid preparation kit

1

Inflammatory Signaling Pathways in Murine Cells

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Lipopolysaccharide (LPS) (L2880, Sigma-Aldrich); Nitric Oxide (NO) assay kit (A013-2-1, Nanjing Jiancheng Bioengineering Institute); Mouse IL-1β ELISA kit (JYM0531Mo, Wuhan Genome Biotechnology Co., Ltd.); BCA Protein Assay Kit (PC0020, Solarbio); SDS-PAGE Gel Rapid Preparation Kit (G2037-50T, Servicebio); anti-PI3K rabbit antibody (AF6241, Affinity), anti-Phospho-AKT1/2/3 rabbit antibody (AF0016, Affinity), anti-AKT1 rabbit antibody (AF0836. Affinity), anti-NF-kB p65 rabbit antibody (AF5006, Affinity), anti-MMP9 rabbit antibody (AF5228, Affinity), anti-TNF alpha rabbit antibody (AF7014, Affinity) and anti-β-actin rabbit antibody (GB11001, Servicebio); HRP-conjugated goat anti-rabbit IgG (GB23204, Servicebio); Nissl staining solution (G1036, Servicebio); Trizol (15596026, ambion); Oligo (dT)18 Primer (3806, TAKARA), PrimeScript II RTase (2690A, TAKARA) and Recombinant RNase Inhibitor (2313A, TAKARA).
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2

Detailed NLRP3 Inflammasome Activation Assay

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Chemicals are listed as follows: A804598 (HY-100483, MedChemExpress Co., Ltd. United States); IL-1Ra (HY-P7029A, MCE, United States); DMSO (D8370, Solarbio, China); Mouse NLRP3 ELISA kit (JYM0765Mo, Wuhan ColorfulGene biological technology Co., Ltd. China); Mouse IL-1β ELISA kit (JYM0531Mo, Wuhan ColorfulGene biological technology Co., Ltd. China); BCA Protein Assay Kit (PC0020, Solarbio, China); SDS-PAGE Gel Rapid Preparation Kit (G2037-50T, Servicebio, China); Recombinant Anti-beta Actin antibody (GB15003, Servicebio, China); NLRP3 Antibody (DF7438, Affinity Biologicals, Canada); Recombinant Anti-P2X7 antibody (ab259942, abcam, United Kingdom); HRP-conjugated goat anti-rabbit IgG (GB23204, Servicebio, China); Anti-Iba1 Rabbit pAb (GB11105, Servicebio, China); Cy3-labeled goat anti-rabbit IgG (GB21303, Servicebio, China); FITC-labeled goat anti-rabbit IgG (GB22303, Servicebio, China); Anti-BrdU Mouse mAb (GB12051, Servicebio, China); Anti-NeuN Rabbit pAb (GB11138, Servicebio, China); HE staining solution (G1005-100ML, Servicebio, China).
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