Edil3

Manufactured by Abcam

Sourced in United States

EDIL3 is a human protein-coding gene that encodes the epidermal growth factor-like protein 3. This gene is involved in the regulation of various cellular processes. The protein functions as an extracellular matrix component.

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Lab products found in correlation

Fitc phalloidin, by Merck Group (1 mentions) Meta laser scanning microscope, by Zeiss (1 mentions) Vegfr2, by Abcam (1 mentions) Vascular endothelial growth factor (vegf), by Abcam (1 mentions) Ripa buffer, by Beyotime (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions) Bca protein assay kit, by Solarbio (1 mentions)

2 protocols using edil3

Visualizing EDIL3 and F-Actin in HCC Cells

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In cell assay, all the HCC cell lines under tests were seeded on cover slides in 24-well plates and incubated overnight. For F-actin staining, cells were incubated with phalloidin-FITC (Sigma-Aldrich) for 75 minutes at room temperature. For EDIL3 staining, cells were incubated with primary antibodies against EDIL3 (Abnova) for 75 minutes at room temperature, followed by an Alexa Fluor 594-conjugated secondary antibody. In tissue staining, two samples embedded in paraffin were subjected to heat-mediated antigen retrieval in pH 6.0 citric acid, and blocked by 10% BSA. The primary antibodies used are EDIL3 (Abnova) and CD31 (Abcam) with Alexa Fluor 594-conjugated secondary antibody against EDIL3 and Fluor 488 against CD31. Immunofluorescence signals were captured using confocal micro-scopy (LSM 510, METALaser Scanning Microscope, Zeiss).

Feng M.X., Ma M.Z., Fu Y., Li J., Wang T., Xue F., Zhang J.J., Qin W.X., Gu J.R., Zhang Z.G, & Xia Q. (2014). Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation. Molecular Cancer, 13, 226.

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Quantitative Protein Analysis by Western Blot

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RIPA buffer (Beyotime, China) and the protease inhibitor PMSF (Solarbio, China) (RIPA: PMSF ratio = 100:1) were used to extract total proteins. The extracted proteins were centrifuged at 13,000 g and 4 °C for 10 min. The BCA protein assay kit (Solarbio, China) was used to quantify the protein content. An automatic protein analyzer (Wes & Jess, USA) was used for the Western blotting assay in accordance with the specific protocols, and β-actin was used as a reference. The blots were then incubated with rabbit anti-human antibodies against β-actin (CST, USA), EDIL3, VEGF, and VEGFR2 (all from Abcam, UK).

Niu X., Li X., Feng Z., Han Q., Li J., Liu Y, & Zhang K. (2023). EDIL3 and VEGF Synergistically Affect Angiogenesis in Endothelial Cells. Clinical, Cosmetic and Investigational Dermatology, 16, 1269-1277.

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