Cell and exosome samples were lysed using RIPA buffer (Beyotime, Shanghai, China) containing protease and phosphatase inhibitors (Epizyme, Shanghai, China). The protein concentration was quanti ed using a BCA protein assay kit (Beyotime). Proteins were separated by 10% (gradient) SDS-PAGE (Epizyme) and transferred onto PVDF membranes (Amersham Hybond 0.45µm; GE Healthcare). The membranes were blocked with 5% milk in trisbuffered saline-Tween (TBS-T) for 2h, then incubated with primary antibodies (anti-CD9(ab92726,Abcam),anti-CD63(ab134045,Abcam), anti-MMP-1(ab134184,Abcam), anti-PAR1(26366-1-AP,Proteintech),anti-Zeb-1(21544-1-AP,Proteintech), anti-Slug(12129-1-AP,Proteintech), anti-SNAI1(13099-1-AP,Proteintech), anti-E-cadherin(20874-1-AP,Proteintech), anti-Vimentin(10366-1-AP,Proteintech), anti-GAPDH(60004-1-Ig,Proteintech)) for 12h at 4°C. the membranes were washed with TBS-T for 3times,15min per time, then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h at RT. The proteins were visualized using the ECL western blotting substrate (cat. WBKLS0100, Millipore) and a Tanon-5200Multi device.
Anti slug 12129 1 ap
Manufactured by Proteintech
Anti-Slug(12129-1-AP) is a primary antibody produced by Proteintech. This antibody is designed for the detection of slug protein in various biological samples. The core function of this product is to provide a tool for researchers to study the expression and localization of slug protein in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Beyotime (2 mentions)
Anti par1 26366 1 ap, by Proteintech (2 mentions)
Anti snai1 13099 1 ap, by Proteintech (2 mentions)
Tanon 5200multi device, by Merck Group (2 mentions)
Anti gapdh 60004 1 ig, by Proteintech (2 mentions)
Ab134045, by Abcam (2 mentions)
Bca protein assay kit, by Beyotime (2 mentions)
Protease and phosphatase inhibitors, by Ipsen (2 mentions)
Ab92726, by Abcam (2 mentions)
Amersham hybond 0.45 m, by GE Healthcare (2 mentions)
Anti zeb 1 21544 1 ap, by Proteintech (2 mentions)
Anti e cadherin 20874 1 ap, by Proteintech (2 mentions)
Anti vimentin 10366 1 ap, by Proteintech (2 mentions)
Hybond, by Cytiva (2 mentions)
2 protocols using anti slug 12129 1 ap
1
Exosome protein profiling by Western blot
2
Exosome protein profiling by Western blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell and exosome samples were lysed using RIPA buffer (Beyotime, Shanghai, China) containing protease and phosphatase inhibitors (Epizyme, Shanghai, China). The protein concentration was quanti ed using a BCA protein assay kit (Beyotime). Proteins were separated by 10% (gradient) SDS-PAGE (Epizyme) and transferred onto PVDF membranes (Amersham Hybond 0.45µm; GE Healthcare). The membranes were blocked with 5% milk in trisbuffered saline-Tween (TBS-T) for 2h, then incubated with primary antibodies (anti-CD9(ab92726,Abcam),anti-CD63(ab134045,Abcam), anti-MMP-1(ab134184,Abcam), anti-PAR1(26366-1-AP,Proteintech),anti-Zeb-1(21544-1-AP,Proteintech), anti-Slug(12129-1-AP,Proteintech), anti-SNAI1(13099-1-AP,Proteintech), anti-E-cadherin(20874-1-AP,Proteintech), anti-Vimentin(10366-1-AP,Proteintech), anti-GAPDH(60004-1-Ig,Proteintech)) for 12h at 4°C. the membranes were washed with TBS-T for 3times,15min per time, then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h at RT. The proteins were visualized using the ECL western blotting substrate (cat. WBKLS0100, Millipore) and a Tanon-5200Multi device.
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