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The CCL-10TM is a cell culture device designed for the maintenance and growth of adherent cell lines. It features a sealed, climate-controlled incubation chamber that provides a stable environment for cell cultures. The device allows for convenient monitoring and manipulation of cell cultures without compromising the controlled conditions.

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CCL-10 (37 citations) ATCC CCL-10 (8 citations) BHK-21 (ATCC CCL-10) (4 citations) BHK-21 (CCL-10) (3 citations) BHK-21 cells (CCL-10; (3 citations)

2 protocols using ccl 10tm

1

SBV Isolate Amplification in BHK-21 Cells

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The SBV stock was obtained through the amplification of the SBV-BH80/11-4 isolate in BHK-21 cells (ATCC®#CCL-10TM).
Bayrou C., Van Laere A.S., Dam Van P., Moula N., Garigliany M.M, & Desmecht D. (2023). Anti-Schmallenberg Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species. Viruses, 15(5), 1055.
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2

WNV Propagation and Titration Protocol

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Transfection was carried out on baby hamster kidney 21 (BHK-21) cells (ATCC®: CCL-10TM) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 5% foetal bovine serum (FBS). Virus stocks were propagated and titrated on Vero E6 cells (ATCC®: CRL-1586TM) cultured in DMEM supplemented with 0.75% sodium bicarbonate, 10 mM hepes buffer and 10% FBS. All media was supplemented with antibiotics (100 U penicillin, 100 µg/mL streptomycin) and 2 mM L-glutamine. The WNV-578/10 strain (GenBank accession number: KC496015) was originally isolated in Hungary from a horse showing clinical signs of encephalitis, which had died in 2010.
Szentpáli-Gavallér K., Lim S.M., Dencső L., Bányai K., Koraka P., Osterhaus A.D., Martina B.E., Bakonyi T, & Bálint Á. (2016). In Vitro and in Vivo Evaluation of Mutations in the NS Region of Lineage 2 West Nile Virus Associated with Neuroinvasiveness in a Mammalian Model. Viruses, 8(2), 49.
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