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2 protocols using palmitoyl sphingomyelin

1

Lipid Composition and Characterization

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1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (DPPS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG), palmitoyl sphingomyelin were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol was purchased from Sigma Aldrich.
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2

Lipids and Fluorescent Probes for Membrane Studies

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Phosphocholine (PC) lipids including DOPC (dioleoyl-PC, or di-18:1-PC), DPPC (dipalmitoyl-PC or di-16:0-PC), POPC (palmitoyl-oleoyl-PC or 16:0-18:1-PC), di16:1-Δ9cis-PC, di18:1-Δ6cis-PC, di14:1-Δ9cis-PC, lyso(18:0)-PC, and palmitoyl sphingomyelin (PSM or 18:1-16:0 SM) were from Avanti Polar Lipids (Alabaster, AL). Texas Red dihexadecanoyl-phosphoethanolamine was from Life Technologies (Grand Island, NY), and cholesterol was from Sigma Aldrich (St. Louis, MO). Lipid structures appear in Fig. S1. Stock solutions of laurdan (Invitrogen, Carlsbad, CA) and C-laurdan (a gift from B. R. Cho, Seoul, Korea) were prepared in ethanol and dimethyl sulfoxide (DMSO). Alcohols (ethanol, propanol, butanol, pentanol, hexanol, octanol, decanol, tetradecanol, hexadecanol, propofol, and 2,6-di-tert-butylphenol), DMSO, and all additional reagents were from Sigma Aldrich unless specified. All alcohols were purchased at their highest available purity. All materials were used as from the manufacturer without further purification.
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