Ramos B cells, an Epstein-Barr virus (EBV)-negative cell line derived from an American Burkitt's lymphoma (17 (link)) and Daudi B cells, an EBV-positive cell line derived from Burkitt's lymphoma (22 (link)), were purchased from the American Type Culture Collection (ATCC; LGC Standards S.r.l, Milan, Italy). Both cell lines were grown in suspension at the optimal cell density of 3×105–1×106 cells/ml in HEPES-buffered RPMI-1640 (Gibco Laboratories Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco), 5 mM glutamine, penicillin/streptomycin (50 IU/ml and 50 μg/ml, respectively). Exponentially growing cells, showing a viability of at least 95%, were administered 3 and 5 Gy at room temperature by a Mevatron M linear accelerator (photonic energy of 6 MV; dose rate 3 Gy/min). Some cellular pools were treated with the NF-κB/IκB pharmacological inhibitor SN50 (Alexis Biochemicals, Lausen, Switzerland) added at the final concentration of 30 μg/ml 45 min before irradiation (23 (link)). Cell cytotoxicity was assayed with trypan blue dye exclusion test and by the evaluation of the cell cycle and apoptosis.
Ramos b cells
Manufactured by American Type Culture Collection
Sourced in Italy
Ramos B cells are a human B lymphoblastoid cell line derived from a patient with Burkitt's lymphoma. The cells are used for various research applications, including the study of B cell biology, signal transduction, and molecular mechanisms involved in B cell development and function.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Expi293f cells, by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
2 protocols using ramos b cells
1
Radiation-Induced Cellular Responses in Burkitt's Lymphoma
2
Cell Culture Protocols for Immune Cells and HEK293 Lines
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THP-1 cells and Ramos B cells (RA 1; American Type Culture Collection [ATCC]) were maintained in RPMI 1640 medium (supplemented with 10% FBS, l -glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin; all from Invitrogen). THP-1 cells were differentiated to macrophages using 10 ng/ml PMA (Sigma-Aldrich) for 12 h, followed by rest for 24 h in complete RPMI 1640 medium. HEK293T cells (ATCC) were maintained in DMEM (supplemented with 10% FBS, l -glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin; all from Invitrogen). Expi293F cells (Thermo Fisher Scientific) were cultured in Expi293 Medium (Life Technologies) at 140 rpm, 37°C, and 8% CO2.
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